Evaluation of two c-ELISA tests for detection of antibodies against PPR in African wildlife

Aman Ullah Guitian J., Waret Agnès, Pfeiffer Dirk, Roger François, Libeau Geneviève, Diallo Adama. 2007. Evaluation of two c-ELISA tests for detection of antibodies against PPR in African wildlife. In : Does control of animal infectious risks offer a new international perspective ? : proceedings of the 12th International Conference of the Association of Institutions of Tropical Veterinary Medicine, Montpellier, France, 20-22 August 2007. Camus Emmanuel (ed.), Cardinale Eric (ed.), Dalibard Christophe (ed.), Martinez Dominique (ed.), Renard Jean-François (ed.), Roger François (ed.). CIRAD. Montpellier : CIRAD, Résumé, 234. ISBN 978-2-87614-650-1 International Conference of the Association of Institutions of Tropical Veterinary Medicine. 12, Montpellier, France, 20 Août 2007/22 Août 2007.

Résumé : Peste des petits ruminants (PPR) is a viral disease affecting both domestic and wild Artiodactyles. Its surveillance and control relies on the use of serological techniques like competitive ELISA. Here we evaluated the performance of c-ELISA-H and c-ELISA-N in wildlife species. We used data from 1244 serum samples obtained from individual animals within 13 African countries that were tested by both, ELISA and VNT (gold standard) in 144 cases. For the prescribed cut-off Sp and Se were estimated as 99.04% (95% CI = 96.19%-100%) and 10.24% (95% CI: 5.53%-16.35%) for c-ELISA-N and 22.72% (95% CI: 14.59-30.95%) and 98.15% (95% CI: 94.55-100%) for c-ELISA-H. ROC curves were obtained to identify cut-offs that would optimize the performance of the tests. New cut-offs were suggected : PI = 21.479 for ELISA-N and PI = 30.45 for c-ELISA-H with new Se and Sp estimates of 74.02% (95% CI: 66.39%-81.64%) and 56.60% (95% CI: 47.17%-66.04%) for c-ELISA-N and 55.54% (95% CI: 45.75%-65.12%) and 72.73% (95% CI: 60.96%-84.50%) for c-ELISA-H respectively. The apparent prevalences of PPR in the studied sites were corrected and 'true' prevalences ranging between 1.45% and 97.85% were obtained. Selection bias as well as lack of blinding were the main limitations of this study. (Texte intégral)

Classification Agris : L73 - Maladies des animaux

Auteurs et affiliations

• Aman Ullah Guitian J., Royal Veterinary College (GBR)
• Waret Agnès, Royal Veterinary College (GBR)
• Pfeiffer Dirk, Royal Veterinary College (GBR)
• Roger François, CIRAD-BIOS-UPR Epidémiologie (FRA) ORCID: 0000-0002-1573-6833
• Libeau Geneviève, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• Diallo Adama, CIRAD-BIOS-UPR Contrôle des maladies (AUT)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/541577/)

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