Ethinylestradiol differentially interferes with IGF-I in liver and extrahepatic sites during development of male and female bony fish

Shved Natallia, Berishvili Giorgi, D'Cotta Helena, Baroiller Jean-François, Segner Helmut, Eppler Elisabeth, Reinecke Manfred. 2007. Ethinylestradiol differentially interferes with IGF-I in liver and extrahepatic sites during development of male and female bony fish. Journal of Endocrinology, 195 : 513-523.

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Growth and sexual development are closely interlinked in fish; however, no reports exist on potential effects of estrogen on the GH/IGF-I-axis in developing fish. We investigate whether estrogen exposure during early development affects growth and the IGF-I system, both at the systemic and tissue level. Tilapia were fed from 10 to 40 days post fertilization (DPF) with 17[alpha]-ethinylestradiol (EE2). At 50, 75, 90, and 165 DPF, length, weight, sex ratio, serum IGF-I (RIA), pituitary GH mRNA and IGF-I, and estrogen receptor [alpha] (ER[alpha]) mRNA in liver, gonads, brain, and gills (real-time PCR) were determined and the results correlated to those of in situ hybridization for IGF-I. Developmental exposure to EE2 had persistent effects on sex ratio and growth. Serum IGF-I, hepatic IGF-I mRNA, and the number of IGF-I mRNA-containing hepatocytes were significantly decreased at 75 DPF, while liver ER[alpha] mRNA was significantly induced. At 75 DPF, a transient decline of IGF-I mRNA and a largely reduced number of IGF-I mRNA-containing neurons were observed in the female brain. In both sexes, pituitary GH mRNA was significantly suppressed. A transient downregulation of IGF-I mRNA occurred in ovaries (75 DPF) and testes (90 DPF). In agreement, in situ hybridization revealed less IGF-I mRNA signals in granulosa and germ cells. Our results show for the first time that developmental estrogen treatment impairs GH/IGF-I expression in fish, and that the effects persist. These long-lasting effects both seem to be exerted indirectly via inhibition of pituitary GH and directly by suppression of local IGF-I in organ-specific cells.

Classification Agris : L52 - Physiologie animale - Croissance et développement
L53 - Physiologie animale - Reproduction

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Shved Natallia, Institute of Anatomy (CHE)
• Berishvili Giorgi, Institute of Anatomy (CHE)
• D'Cotta Helena, CIRAD-PERSYST-UPR Aquaculture (FRA) ORCID: 0000-0003-2739-3476
• Baroiller Jean-François, CIRAD-PERSYST-UPR Aquaculture (FRA) ORCID: 0000-0002-0992-1725
• Segner Helmut, Centre for Fish and Wildlife Health (CHE)
• Eppler Elisabeth, Institute of Anatomy (CHE)
• Reinecke Manfred, Institute of Anatomy (CHE)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/542105/)

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