Musa genetic mapping

Hippolyte Isabelle, Seguin Marc, Bakry Frédéric, Gardes Laëtitia, Baurens Franc-Christophe, Miller Roberto Neil Gerard, Khan Imtiaz A., Jenny Christophe, Carreel Françoise, Huttner Eric, Perrier Xavier, Kilian Andrzej, Risterucci Ange-Marie, Glaszmann Jean-Christophe. 2008. Musa genetic mapping. In : Abstracts of Plant and Animal Genomes XVIth Conference, San Diego, CA (USA), January 12-16, 2008. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 16, San Diego, États-Unis, 12 Janvier 2008/16 Janvier 2008.

Résumé : We report a reference map of Musa acuminata from 180 individuals of BORLI population. This F1 population derived from the cross between M.a. "Borneo" x M.a. "Pisang Lilin" which was chosen for mapping mainly because of the great heterozygosity of both parents (76 % each). For map construction we genotyped 180 SSR markers on 180 individuals, 12 RGA-RFLP probes on 112 individuals and 380 DArT markers on 92 individuals. Using the software Joinmap 4 at LOD 5, Kosambi calculation distance and the regression mapping algorithm, we defined 11 main linkage groups, as expected, on Borneo parent and 10 on Pisang lilin due to one translocation event in the male parent. Despite structural differences between the parents, a consensus map was built. In order to check genotyping results and to analyse structural rearrangements, we developed an original method using Neighbor Joining algorithm implemented in the genetic diversity software Darwin5. It allows visualizing graphically disruptions in alignments arising from these structural rearrangements. (Texte intégral)

Mots-clés Agrovoc : Musa acuminata

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Hippolyte Isabelle, CIRAD-BIOS-UPR Multiplication végétative (FRA)
• Seguin Marc, CIRAD-BIOS-UMR DAP (FRA)
• Bakry Frédéric, CIRAD-BIOS-UPR Multiplication végétative (FRA)
• Gardes Laëtitia ORCID: 0000-0003-1787-1331
• Baurens Franc-Christophe, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0002-5219-8771
• Miller Roberto Neil Gerard, UCB (BRA)
• Khan Imtiaz A., Nuclear Institute of Agriculture (PAK)
• Jenny Christophe, CIRAD-BIOS-UPR Multiplication végétative (GLP) ORCID: 0000-0001-6165-6888
• Carreel Françoise, CIRAD-BIOS-UPR Multiplication végétative (FRA)
• Huttner Eric, UCB (BRA)
• Perrier Xavier, CIRAD-BIOS-UPR Multiplication végétative (FRA) ORCID: 0000-0002-0092-003X
• Kilian Andrzej, UCB (BRA)
• Risterucci Ange-Marie, CIRAD-BIOS-UMR DAP (FRA)
• Glaszmann Jean-Christophe, CIRAD-BIOS-DIR (FRA) ORCID: 0000-0001-9918-875X

Source : Cirad - Agritrop (https://agritrop.cirad.fr/545521/)

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