Development of molecular tools for studies in the Calliptamus genus

Blanchet Elodie, Risterucci Ange-Marie, Billot Claire, Chapuis Marie-Pierre, Blondin Laurence, Rivallan Ronan, Pagès Christine, Foucart Antoine, Vassal Jean-Michel, Lecoq Michel. 2009. Development of molecular tools for studies in the Calliptamus genus. Metaleptea (Special Meeting), Résumé : 110-111. International Congress of Orthopterology. 10, Antalya, Turquie, 21 Juin 2009/25 Juin 2009.

Résumé : The genus Calliptamus (Orthoptera, Acrididae) includes locusts and grasshoppers, spread mainly throughout the Mediterranean Basin and as far as the southern part of Siberia. Some species are of substantial economic importance, such as C. italicus (Linné, 1758). Despite many studies on their taxonomy and biology, the identification of many species remains difficult, particularly for females and nymphs. Furthermore, in spite of their economic importance, dispersion capabilities which could have an impact in outbreak as demonstrated for other locusts - are not well understood. In order to clarify both aspects - identification and dispersion capabilities - we tried to develop molecular tools for various species of this genus distributed in Southern France. We focused our studies on C. italicus, C. barbarus (Costa, 1836) and C. wattenwylianus (Pantel, 1896). In order to facilitate the identification process, we developed a multiplex PCR assays to discriminate - quickly and inexpensively - any specimen collected in the field at the species level. Then, we developed new microsatellite markers to be used for further studies on dispersion capabilities. Seven markers were obtained for C. barbarus, six for C. italicus, three of them cross amplified on C. wattenwylianus. Genetic analyses were performed on two sampling seasons, at different geographic scales: the first study at an individual level (on two sites of 72 km² and 40 km apart, in Southern France), the second at a population level at different localities throughout the Mediterranean Basin. Analyses are in progress and results will be provided during the congress. (Texte intégral)

Mots-clés Agrovoc : Calliptamus, identification, dynamique des populations, marqueur génétique, microsatellite, PCR

Mots-clés géographiques Agrovoc : France

Mots-clés complémentaires : Calliptamus barbarus, Calliptamus italicus, Calliptamus wattenwylianus

Classification Agris : H10 - Ravageurs des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Blanchet Elodie, CIRAD-BIOS-UPR Acridologie (FRA)
• Risterucci Ange-Marie, CIRAD-BIOS-UMR DAP (FRA)
• Billot Claire, CIRAD-BIOS-UMR DAP (FRA)
• Chapuis Marie-Pierre, CIRAD-BIOS-UPR Acridologie (AUS)
• Blondin Laurence, CIRAD-BIOS-UPR Acridologie (FRA)
• Rivallan Ronan, CIRAD-BIOS-UMR DAP (FRA)
• Pagès Christine, CIRAD-BIOS-UPR Acridologie (FRA)
• Foucart Antoine, CIRAD-BIOS-UPR Acridologie (FRA)
• Vassal Jean-Michel, CIRAD-BIOS-UPR Acridologie (FRA)
• Lecoq Michel, CIRAD-BIOS-UPR Acridologie (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/550545/)

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