Genetic diverstiy of Sugarcane yellow leaf virus in a sugarcane selection plot in Guadeloupe (FWI)

Girard Jean-Claude, Fernandez Emmanuel, Daugrois Jean-Heinrich, Roques Danièle, Roumagnac Philippe, Rott Philippe. 2010. Genetic diverstiy of Sugarcane yellow leaf virus in a sugarcane selection plot in Guadeloupe (FWI). In : ISSCT 2010: 27th Congress of the International Society of Sugar Cane Technologists Congres, Veracruz, Mexique, 7-11 mars 2010. ISSCT. s.l. : s.n., 9 p. ISSCT Congress. 27, Veracruz, Mexique, 7 March 2010/11 March 2010.

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Titre français : Diversité génétique du Sugarcane yellow leaf virus dans une parcelle de sélection de canne à sucre en Guadeloupe / Titre espagnol : Diversidad genetica del Sugarcane yellow leaf virus en una parcela de seleccion de caña de azucar en Guadeloupe (FWI)

Abstract : THE GENETIC diversity of Sugarcane yellow leaf virus (SCYLV) was investigated in a sugarcane selection plot in Guadeloupe to determine the incidence of the different known virus genotypes (BRA-PER, CUB and REU) in a breeding progeny. Four F1 leaves were randomly collected from each of 154 sugarcane clones and tested for the presence of SCYLV by tissue blot immunoassay (TBIA). The leaf samples were stored at ¿80°C until total RNA was extracted from SCYLV-infected leaves. The virus genotypes were determined by RT-PCR and primer pairs specific to each virus genotype. Eighty-two percent of the tested leaves were infected by SCYLV and all known virus genotypes occurred in the selection plot. The majority of plants were infected by genotypes CUB or REU, or by a mixture of these two genotypes. This situation was completely different from the situation observed in commercial fields in Guadeloupe where the incidence of SCYLV is much lower and where most plants are infected by genotype REU. The significance of this striking situation will be further investigated. (Résumé d'auteur)

Mots-clés Agrovoc : Saccharum officinarum, Variation génétique, Virus des végétaux

Mots-clés géographiques Agrovoc : Guadeloupe

Mots-clés complémentaires : Sugarcane yellow leaf virus

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Auteurs et affiliations

  • Girard Jean-Claude, CIRAD-BIOS-UMR BGPI (FRA)
  • Fernandez Emmanuel, CIRAD-BIOS-UMR BGPI (FRA)
  • Daugrois Jean-Heinrich, CIRAD-BIOS-UPR Multiplication végétative (GLP)
  • Roques Danièle, CIRAD-BIOS-UPR Multiplication végétative (GLP)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039
  • Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159

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