Endogenous caulimovirid sequences are widespread in plant genomes

Geering Andrew D., Choisne Nathalie, Vezzulli Silvia, Sharahskin Tanya, Velasco Riccardo, Quesneville Hadi, Teycheney Pierre-Yves. 2010. Endogenous caulimovirid sequences are widespread in plant genomes. In : EMBO Workshop Genomic Approaches to Interactions between Plant Virus, their Hosts and their Vectors, 12-16 June 2010, Fenestrelle, Italy. s.l. : s.n., Résumé, 1 p. EMBO Workshop Genomic Approaches to Interactions between Plant Virus, their Hosts and their Vectors, Fenestrelle, Italie, 12 Juin 2010/16 Juin 2010.

Résumé : Endogenous sequences from members of the Caulimoviridae and Geminiviridae families have been identified in the genome of several plant species. They are thought to result from illegitimate recombination events and are generally replication-defective. However, some caulimovirid sequences of tobacco, petunia and banana are capable of causing infection. We performed in silico analyses on nucleotide sequences from plant genome databases and reconstituted 11 full length and potentially infectious viral genomes from endogenous viral sequences embedded in the genomes of monocotyledonous and dicotyledonous Brassicaceae, Euphorbiacae, Fabacae, Myrtaceae, Poaceae, Rutaceae, Saliaceae and Vitaceae plant species. Sequence comparisons show that the corresponding viruses belong to a new genus in the family Caulimoviridae, tentatively named Dionyvirus. Mapping of endogenous Dionyvirus sequences was achieved in two fully sequenced grape genomes, allowing for the first time the study of the distribution pattern of endogenous viral sequences at the host plant genome scale. Dionyvirus-specific primers were designed and used for a PCR-based large scale screening of plant germplasm. It showed that endogenous Dionyvirus sequences are widespread among plants of temperate, tropical and arctic origins, and belong to distinct viral species. Endogenous Dionyvirus sequences belonging to distinct viral species were also identified in single host plants, showing that endogenization of viral sequences is a common phenomenon in plants. Our work shed new lights on plant/virus molecular interactions. The potential contributions of endogenous viral sequences to normal plant functions and to plant and virus evolution will be discussed. (Texte intégral)

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Geering Andrew D., QDPI (AUS)
• Choisne Nathalie, INRA (FRA)
• Vezzulli Silvia, IASMA (ITA)
• Sharahskin Tanya, Queensland University of Technology (AUS)
• Velasco Riccardo, IASMA (ITA)
• Quesneville Hadi, INRA (FRA)
• Teycheney Pierre-Yves, CIRAD-BIOS-UPR Multiplication végétative (GLP) ORCID: 0000-0002-9754-0745

Source : Cirad - Agritrop (https://agritrop.cirad.fr/556017/)

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