Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the threatened murine rodent, Leopoldamys neilli

Latinne Alice, Waengsothorn Surachit, Risterucci Ange-Marie, Michaux Johan. 2011. Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the threatened murine rodent, Leopoldamys neilli. Conservation Genetics Resources, 3 (3) : 511-513.

Quartile : Q4, Sujet : BIODIVERSITY CONSERVATION / Quartile : Q4, Sujet : GENETICS & HEREDITY

Résumé : Leopoldamys neilli is a threatened murine rodent species endemic to limestone karsts of Thailand. Twelve microsatellite loci were identified using the method of microsatellite-enriched libraries. Polymorphism was assessed in samples (N = 62) from four geographically distinct populations in Thailand. Number of alleles per locus ranged from 9 to 15 (average 11.6). Observed and expected heterozygosities varied from 0.28 to 1.0 and from 0.44 to 0.91, respectively. There was no evidence for linkage disequilibrium, however, four loci showed evidence of departure from Hardy-Weinberg equilibrium in one population. Presence of null alleles was not detected in all the 12 loci. These first microsatellites primers developed for L. neilli will provide information on the fine-scale genetic structure of this threatened species and will help in the development of future conservation policies.

Mots-clés Agrovoc : Rodentia, rongeur, microsatellite, marqueur génétique, PCR

Mots-clés géographiques Agrovoc : Asie du Sud-Est, Thaïlande

Mots-clés complémentaires : Leopoldamys neilli

Classification Agris : L10 - Génétique et amélioration des animaux
L60 - Taxonomie et géographie animales

Champ stratégique Cirad : Axe 6 (2005-2013) - Agriculture, environnement, nature et sociétés

Auteurs et affiliations

• Latinne Alice, Université de Liège (BEL)
• Waengsothorn Surachit, TISTR (THA)
• Risterucci Ange-Marie, CIRAD-BIOS-UMR AGAP (FRA)
• Michaux Johan, Montpellier SupAgro (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/560506/)

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