The auxin signalling network translates dynamic input into robust patterning at the shoot apex

Vernoux Teva, Brunoud Géraldine, Farcot Etienne, Morin Valérie, Van Den Daele Hilde, Legrand Jonathan, Oliva Marina, Das Pradeep, Larrieu Antoine, Wells Darren, Guédon Yann, Armitage Lynne, Picard Franck, Guyomarc'h Soizic, Cellier Coralie, Parry Geraint, Koumproglou Rachil, Doonan John H., Estelle Mark, Godin Christophe, Kepinski Stefan, Bennett Malcolm J., De Veylder Lieven, Traas Jan. 2011. The auxin signalling network translates dynamic input into robust patterning at the shoot apex. Molecular Systems Biology (7):508, 15 p.

Quartile : Outlier, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : The plant hormone auxin is thought to provide positional information for patterning during development. It is still unclear, however, precisely how auxin is distributed across tissues and how the hormone is sensed in space and time. The control of gene expression in response to auxin involves a complex network of over 50 potentially interacting transcriptional activators and repressors, the auxin response factors (ARFs) and Aux/IAAs. Here, we perform a large-scale analysis of the Aux/IAA-ARF pathway in the shoot apex of Arabidopsis, where dynamic auxin-based patterning controls organogenesis. A comprehensive expression map and full interactome uncovered an unexpectedly simple distribution and structure of this pathway in the shoot apex. A mathematical model of the Aux/IAA-ARF network predicted a strong buffering capacity along with spatial differences in auxin sensitivity.We then tested and confirmed these predictions using a novel auxin signalling sensor that reports input into the signalling pathway, in conjunction with the published DR5 transcriptional output reporter. Our results provide evidence that the auxin signalling network is essential to create robust patterns at the shoot apex.

Mots-clés Agrovoc : développement biologique, auxine, Arabidopsis

Classification Agris : F62 - Physiologie végétale - Croissance et développement
U10 - Informatique, mathématiques et statistiques

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

• Vernoux Teva, ENS Lyon (FRA)
• Brunoud Géraldine, ENS Lyon (FRA)
• Farcot Etienne, INRIA (FRA)
• Morin Valérie, ENS Lyon (FRA)
• Van Den Daele Hilde, Université de Gand (BEL)
• Legrand Jonathan, INRIA (FRA)
• Oliva Marina, ENS Lyon (FRA)
• Das Pradeep, ENS Lyon (FRA)
• Larrieu Antoine, University of Nottingham (GBR)
• Wells Darren, University of Nottingham (GBR)
• Guédon Yann, CIRAD-BIOS-UMR AGAP (FRA)
• Armitage Lynne, University of Leeds (GBR)
• Picard Franck, CNRS (FRA)
• Guyomarc'h Soizic, ENS Lyon (FRA)
• Cellier Coralie, ENS Lyon (FRA)
• Parry Geraint, Indiana University (USA)
• Koumproglou Rachil, John Innes Centre (GBR)
• Doonan John H., John Innes Institute (GBR)
• Estelle Mark, Indiana University (USA)
• Godin Christophe, INRIA (FRA)
• Kepinski Stefan, University of Leeds (GBR)
• Bennett Malcolm J., University of Nottingham (GBR)
• De Veylder Lieven, Université de Gand (BEL)
• Traas Jan, ENS Lyon (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/560833/)

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