Microsatellite diagnosis of species within the Ceratitis fasciventris , C. anaonae, C.rosa species complex (Diptera : Tephritidae)

Virgilio Massimiliano, Delatte Hélène, Backeljau Thierry, Quilici Serge. 2012. Microsatellite diagnosis of species within the Ceratitis fasciventris , C. anaonae, C.rosa species complex (Diptera : Tephritidae). In : 2nd CRP meeting Cryptic species, Brisbane, 2012. s.l. : s.n., Diaporama, (29 vues) FAO-IAEA RCM on "Resolution of cryptic species complexes of Tephritid pest to overcome constraints to SIT application and international trade". 2, Brisbane, Australie, 30 Janvier 2012/3 Février 2012.

Résumé : Specimens of Ceratitis fasciventris, C. anonae, and C. rosa (FAR) were collected across the African distribution of the three species and genotyped at 16 polymorphic microsatellite markers (27 populations, n=621). Genetic distance analyses allow distinguishing at least five well-supported population groups, each including samples from one of the three morphospecies. The Bayesian assignments implemented in STRUCTURE show that : (1) C. rosa is represented by at least two clusters of individuals (R1, R2) that can occur in sympatry/parapatry, (2) C. fasciventris is also represented by at least two clusters (F1, F2) that appear to be spatially segregated, and (3) C. anonae is genetically more homogeneous and doesn.t show a clear intraspecific structuring (cluster A).Genetic divergences between the C. rosa clusters, as well as between the C. fasciventris clusters are comparable to the interspecific divergences observed among C. fasciventris, C. anonae, and C. rosa. Stronger genetic differentiation occurs between the morphologically similar C. rosa and C. fasciventris, while C. anonae appears as closely related to both F1 and R2. Remarkably, C. rosa represents 99.0% and 97.0% of specimens assigned to clusters R1 and R2, C. fasciventris 100.0% and 98.7% of specimens assigned to clusters F1 and F2 and C. anonae 95.4% of specimens assigned to cluster A.Microsatellite markers appear thus to be a valuable starting point in the molecular diagnosis of the FAR complex.(résumé d'auteur)

Classification Agris : H10 - Ravageurs des plantes

Auteurs et affiliations

• Virgilio Massimiliano, Musée royal de l'Afrique centrale (BEL)
• Delatte Hélène, CIRAD-BIOS-UMR PVBMT (REU)
• Backeljau Thierry, University of Antwerp (BEL)
• Quilici Serge, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/563610/)

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