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Aeschynomene evenia, a model plant for studying the molecular genetics of the nod-independent rhizobium-legume symbiosis

Arrighi Jean-François, Cartieaux Fabienne, Brown Spencer, Rodier-Goud Marguerite, Boursot Marc, Fardoux Joël, Patrel Delphine, Gully Djamel, Fabre Sandrine, Chaintreuil Clémence, Giraud Eric. 2012. Aeschynomene evenia, a model plant for studying the molecular genetics of the nod-independent rhizobium-legume symbiosis. Molecular Plant-Microbe Interactions, 25 (7) : pp. 851-861.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Research on the nitrogen-fixing symbiosis has been focused, thus far, on two model legumes, Medicago truncatula and Lotus japonicus, which use a sophisticated infection process involving infection thread formation. However, in 25% of the legumes, the bacterial entry occurs more simply in an intercellular fashion. Among them, some Aeschynomene spp. are nodulated by photosynthetic Bradyrhizobium spp. that do not produce Nod factors. This interaction is believed to represent a living testimony of the ancestral state of the rhizobium-legume symbiosis. To decipher the mechanisms of this Nod-independent process, we propose Aeschynomene evenia as a model legume because it presents all the characteristics required for genetic and molecular analysis. It is a short-perennial and autogamous species, with a diploid and relatively small genome (2n = 20; 460 Mb/1C). A. evenia 'IRFL6945' is nodulated by the well-characterized photosynthetic Bradyrhizobium sp. strain ORS278 and is efficiently transformed by Agrobacterium rhizogenes. Aeschynomene evenia is genetically homozygous but polymorphic accessions were found. A manual hybridization procedure has been set up, allowing directed crosses. Therefore, it should be relatively straightforward to unravel the molecular determinants of the Nod-independent process in A. evenia. This should shed new light on the evolution of rhizobium-legume symbiosis and could have important agronomic implications. (Résumé d'auteur)

Mots-clés Agrovoc : Aeschynomène

Classification Agris : F60 - Plant physiology and biochemistry
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Arrighi Jean-François, IRD (FRA)
  • Cartieaux Fabienne, IRD (FRA)
  • Brown Spencer, CNRS (FRA)
  • Rodier-Goud Marguerite, CIRAD-DG-EVALUATION (FRA)
  • Boursot Marc, IRD (FRA)
  • Fardoux Joël, IRD (FRA)
  • Patrel Delphine, IRD (FRA)
  • Gully Djamel, IRD (FRA)
  • Fabre Sandrine, CIRAD-BIOS-UMR RPB (FRA)
  • Chaintreuil Clémence, IRD (FRA)
  • Giraud Eric, IRD (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/564533/)

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