Relationship between parental chromosomic contribution and nuclear DNA content in the coffee interspecific hybrid C. pseudozanguebariae x C. liberica var 'dewevrei'

Barré Philippe, Layssac Marion, D'Hont Angélique, Louarn Jacques, Charrier André, Hamon Serge, Noirot Michel. 1998. Relationship between parental chromosomic contribution and nuclear DNA content in the coffee interspecific hybrid C. pseudozanguebariae x C. liberica var 'dewevrei'. Theoretical and Applied Genetics, 96 (2) : 301-305.

Résumé : F 1 hybrids were obtained between two co¤ee species with the same chromosome number (2n"22) but with di¤erent nuclear DNA contents [C. pseudozanguebariae (PSE) 2C"1.13 pg and C. liberica var ÔdewevreiÕ (DEW) 2C"1.42 pg]. G2 hybrids were obtained by open-pollination of the F 1 hybrids. Genomic in situ hybridisation (GISH) and ßow cytometry were used on six F 1 hybrids and seven G2 hybrids to determine their parental chromosomic contribution and their nuclear DNA content (qDNA), respectively. GISH e¦ciently identiÞed chromosomes from both species. F 1 hybrids had a qDNA intermediate between that of the parental species and contained the expected 11 chromosomes from each species. There was a linear relationship between the number of PSE chromosomes and the nuclear DNA content, which indicates that ßow cytometry can be used to give a rough estimate of the parental chromosomic contribution in G2 hybrids.

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Barré Philippe, ORSTOM [Institut français de recherche scientifique pour le développement en coopération] (FRA)
• Layssac Marion, ORSTOM [Institut français de recherche scientifique pour le développement en coopération] (FRA)
• D'Hont Angélique, CIRAD-CA-CAPA (FRA)
• Louarn Jacques
• Charrier André, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
• Hamon Serge, ORSTOM [Institut français de recherche scientifique pour le développement en coopération] (FRA)
• Noirot Michel, ORSTOM [Institut français de recherche scientifique pour le développement en coopération] (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/564605/)

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