Molecular identification of palaearctic Culicoides blood meals from different localities in France

Garros Claire, Gardes Laëtitia, Viennet Elvina, Allene Xavier, Rakotoarivony Ignace, Balenghien Thomas. 2010. Molecular identification of palaearctic Culicoides blood meals from different localities in France. In : Proceedings of the 17th European Society for Vector Ecology Conference, 13th - 17th September 2010 (ESOVE 2010), Wroclaw, Poland : Conference Programme and abstract book. Eds. Katarzyna Rydzanicz, Elzbieta Lonc. s.l. : s.n., 58. ISBN 978-83-60425-53-4 European Society for Vector Ecology Conference. 17, Wroclaw, Pologne, 13 Septembre 2010/17 Septembre 2010.

Résumé : Culicoides are small biting midges involved world wide in bluetongue and African horse sickness transmission. Feeding behaviour as well as spatiotemporal dynamics of the trophic behaviour of Culicoides is unknown at the specifie level for palaearctic species. Multiplex allele-specific peR assays were used to identify the blood meal source of five main palaearctic species. Species-specific primers of potential hosts (cow, goat, sheep, human, cat, dog, horse) were derived from vertebrate mitochondrial cytochrome b. Two different multiplex assays allowed the identification of blood meals from livestock or domestic fauna. It also enabled us to identify whether multiple blood meals had been taken (gonotrophic concordance). Moreover, this me\hod allows identifying specimens at the specifie level. The first results highlight the interest of this valu able tool combined with species identification assays, and suggest that Culicoides species may have an opportunistic behaviour regarding the host distribution and density. Future studies will focus on the temporal dynamics of trophic behaviour.

Classification Agris : L72 - Organismes nuisibles des animaux
L73 - Maladies des animaux

Auteurs et affiliations

• Garros Claire, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0003-4378-5031
• Gardes Laëtitia, IRD (FRA) ORCID: 0000-0003-1787-1331
• Viennet Elvina, CIRAD-BIOS-UMR CMAEE (FRA)
• Allene Xavier, CIRAD-BIOS-UMR CMAEE (FRA)
• Rakotoarivony Ignace, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0009-0002-3480-6734
• Balenghien Thomas, CIRAD-BIOS-UMR CMAEE (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/569471/)

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