Molecular responses of coffee plants to drought stress : S04T04

Marraccini Pierre, Vieira Natalia Gomes, Duarte Karoline Estefani, Aquino S.O., Carneiro Fernanda A, Costa Tatiana S., Sujii Patricia. S, Vinecky Felipe, Alekcevetch Jean Carlos, Alves Gabriel Sergio Costa, Leroy Thierry, De Bellis Fabien, Ferrão Maria Amélia G., Damatta Fabio M., Pot David, Silva Vânia Aparecida, Rodrigues Geraldo Stachetti, Andrade Alan Carvalho. 2013. Molecular responses of coffee plants to drought stress : S04T04. In : Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century : Book of abstracts of the CIBA 2013. Brasileiro Ana Christina Miranda (ed.), Fortes Ferreira Claudia (ed.), Fernandez Diana (ed.), Micheli Fabienne (ed.), Coelho Filho M.A. (ed.), Marraccini Pierre (ed.). EMBRAPA, UESC, CIRAD, IRD. Brasilia : EMBRAPA, Résumé, p. 29. Workshop on Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century, Ilhéus-Bahia, Brésil, 6 November 2013/8 November 2013.

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Abstract : Drought is a key factor affecting coffee plant development and production. In the context of global warming, the generation of drought-tolerant coffee varieties has now turned into one of the priorities of many coffee research institutes. At the genetic level, it is well known that variability exists within the Coffea genus regarding the tolerance to drought-tolerant. During the last decade, several drought-tolerant clones of C. canephora Conilon have been characterized as vigorous plants with high productivity throughout years under drought stress. Physiological analyses suggested that drought tolerance could be a direct consequence of better root development or of enhanced activity of antioxidant enzymes. The recent advances in coffee genomics mainly expressed sequence tag (EST) sequencing projects now open the way to study the molecular and genetic determinism of drought tolerance and to the identification of molecular markers that could be used to speed up coffee breeding programs. With the aim to investigate the molecular mechanisms underlying drought tolerance in coffee plants of C. canephora and C. arabica, qPCR experiments identified more than 80 candidate genes (CGs) presenting differential gene expression between drought-tolerant and drought-susceptible clones/cultivars cultivated under different (with or without) irrigation conditions. Based on the results obtained in C. canephora, we concluded that factors involved a complex network of responses probably involving the abscisic (ABA) signaling pathway and nitric oxide (NO) are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed in drought-tolerant clones. In the drought-tolerant I59 cultivar C. arabica, many CGs involved in the signal transduction pathway of drought stress but also in the synthesis of several biochemical compounds (derivative-sugars etc...), were highly over-expressed under drought compared to drought-susceptible Rubi cultivar. We also identified several CGs that do not exhibit any similarity with those already deposited in global databases and commonly called "no hits". Recent concepts, called these "no hits" as "orphan genes" and postulate that the emergence of these are the result of adaptive responses specific to each species as a function of stresses and adverse conditions faced by these plants during the evolutionary process. This work presents data of expression profiles obtained for several CGs and some orphan genes (called CcUnk [Unknown]) and discussed their putative role in coffee responses to abiotic stress. Work supported by CAPES-COFECUB, CIRAD, Consórcio Pesquisa Café and INCT-Café (CNPq/FAPEMIG). (Texte intégral)

Classification Agris : F30 - Plant genetics and breeding
H50 - Miscellaneous plant disorders

Auteurs et affiliations

  • Marraccini Pierre, CIRAD-BIOS-UMR AGAP (BRA) ORCID: 0000-0001-7637-6811
  • Vieira Natalia Gomes, EMBRAPA (BRA)
  • Duarte Karoline Estefani, EMBRAPA (BRA)
  • Aquino S.O., EMBRAPA (BRA)
  • Carneiro Fernanda A, EMBRAPA (BRA)
  • Costa Tatiana S., EMBRAPA (BRA)
  • Sujii Patricia. S, EMBRAPA (BRA)
  • Vinecky Felipe, EMBRAPA (BRA)
  • Alekcevetch Jean Carlos, EMBRAPA (BRA)
  • Alves Gabriel Sergio Costa, EMBRAPA (BRA)
  • Leroy Thierry, CIRAD-BIOS-UMR AGAP (FRA)
  • De Bellis Fabien, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-7070-7691
  • Ferrão Maria Amélia G., INCAPER (BRA)
  • Damatta Fabio M., EPAMIG (BRA)
  • Pot David, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6144-8448
  • Silva Vânia Aparecida, EPAMIG (BRA)
  • Rodrigues Geraldo Stachetti, EMBRAPA (FRA)
  • Andrade Alan Carvalho, EMBRAPA (BRA)

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