West Nile virus surveillance, Brazil, 2008-2010

Ometto Tatiana, Durigon Edison Luiz, De Araujo Jansen, Aprelon Rosalie, Moura de Aguiar Daniel, Cavalcante Cuacyara Tenorio, Melo Rosane Marini, Levi José Eduardo, Mendes de Azevedo Júnio Severino, Petry Maria Virgínia, Neto Isaac Simão, Serafini Patrícia, Villalobos Eliana, Cunha Elenice Maria Sequetin, Lara Maria do Carmo Custódio S. H., Ferreira Dales Nava Alessandra, Schiavo Nardi Marcello, Hurtado Renata, Rodrigues Roberta B., Sherer Angelo Luís, Sherer Janete de Fátima Martins, Geraldi Marcelo Plaisant, Moraes de Seixas Marina Maria, Peterka Cassio, De Souza Bandeira Debora, Pradel Jennifer, Vachiery Nathalie, Labruna Marcelo Bahia, Aranha de Camargo Luiz Marcelo, Lanciotti Robert, Lefrançois Thierry. 2013. West Nile virus surveillance, Brazil, 2008-2010. Transactions of the Royal Society of Tropical Medicine and Hygiene, 107 (11) : 723-730.

Quartile : Q2, Sujet : TROPICAL MEDICINE / Quartile : Q2, Sujet : PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH (Science)

Résumé : Background West Nile virus (WNV) is an emergent pathogen that is widely distributed in North and Central America. The recent introduction in South America has focused attention on the spread of WNV across Southern American countries. The transmission network involves mosquitoes, birds, horses and humans. Methods The serological evaluation of sera from 678 equids and 478 birds was performed using a WNV-specific blocking ELISA, and only the positive results were confirmed by plaque reduction neutralisation tests (PRNTs). Molecular analysis was performed on sera from 992 healthy equids and on 63 macerates of brains from equids that died of encephalitis and had previously tested negative for other pathogens. We also tested swabs from 928 birds. The samples analysed were collected in different biomes of Brazil. Results We identified WNV antibodies by ELISA in thirteen equids and five birds, and PRNT90 confirmed WNV positivity in four equid samples collected in 2009 in an area between the Amazon and the Pantanal. None of the ELISA positive bird samples were confirmed by PRNT90, and all samples tested by RT-PCR were negative. Conclusion WNV circulation is confirmed by this large scale survey even in the absence of detection of clinical cases.

Mots-clés Agrovoc : fièvre, flavivirus, surveillance épidémiologique, sérologie, biologie moléculaire, Equidae, oiseau, migration animale, Enquête pathologique, technique immunologique, sérum sanguin, Test ELISA

Mots-clés géographiques Agrovoc : Brésil

Mots-clés complémentaires : Fièvre du Nil occidental

Classification Agris : L73 - Maladies des animaux
U30 - Méthodes de recherche
L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Ometto Tatiana, USP (BRA)
• Durigon Edison Luiz, USP (BRA)
• De Araujo Jansen, USP (BRA)
• Aprelon Rosalie, CIRAD-BIOS-UMR CMAEE (GLP)
• Moura de Aguiar Daniel, UFMT (BRA)
• Cavalcante Cuacyara Tenorio, USP (BRA)
• Melo Rosane Marini, Instituto de Defesa Agropecuária do Estado do Mato Grosso (BRA)
• Levi José Eduardo, USP (BRA)
• Mendes de Azevedo Júnio Severino, UFRPE (BRA)
• Petry Maria Virgínia, Universidade do Vale do Rio dos Sinos (BRA)
• Neto Isaac Simão, Centro Nacional de Pesquisa e Conservação de Aves Silvestres (BRA)
• Serafini Patrícia, Centro Nacional de Pesquisa e Conservação de Aves Silvestres (BRA)
• Villalobos Eliana, Centro de Pesquisa e Desenvolvimento de Sanidade Animal (BRA)
• Cunha Elenice Maria Sequetin, Instituto Alberto Luiz Coimbra de pos-graduacao e pesquisa de engenharia (BRA)
• Lara Maria do Carmo Custódio S. H., Centro de Pesquisa e Desenvolvimento de Sanidade Animal (BRA)
• Ferreira Dales Nava Alessandra, EcoHealth Alliance (BRA)
• Schiavo Nardi Marcello, Centro Nacional de Pesquisa e Conservação de Aves Silvestres (BRA)
• Hurtado Renata, USP (BRA)
• Rodrigues Roberta B., UFRPE (BRA)
• Sherer Angelo Luís, Universidade do Vale do Rio dos Sinos (BRA)
• Sherer Janete de Fátima Martins, Universidade do Vale do Rio dos Sinos (BRA)
• Geraldi Marcelo Plaisant, USP (BRA)
• Moraes de Seixas Marina Maria, USP (BRA)
• Peterka Cassio, Centro Nacional de Pesquisa e Conservação de Aves Silvestres (BRA)
• De Souza Bandeira Debora, Centro Nacional de Pesquisa e Conservação de Aves Silvestres (BRA)
• Pradel Jennifer, CIRAD-BIOS-UMR CMAEE (GLP)
• Vachiery Nathalie, CIRAD-BIOS-UMR CMAEE (GLP)
• Labruna Marcelo Bahia, USP (BRA)
• Aranha de Camargo Luiz Marcelo, USP (BRA)
• Lanciotti Robert, CDC (USA)
• Lefrançois Thierry, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0001-8793-5228

Source : Cirad - Agritrop (https://agritrop.cirad.fr/571933/)

Métriques

[ Page générée et mise en cache le 2024-01-28 ]