Investigating chromosomal structural variations in Musa acuminate using NGS approaches : [W077]

Martin Guillaume, Carreel Françoise, Baurens Franc-Christophe, Cardi Céline, Kilian Andrzej, Rouard Mathieu, Jenny Christophe, D'Hont Angélique. 2014. Investigating chromosomal structural variations in Musa acuminate using NGS approaches : [W077]. In : Plant and Animal Genomes Conference XXII Conference, San diego, United States, San Diego, United States, January 11-15, 2014. s.l. : s.n., Résumé Plant and Animal Genome Conference. 22, San Diego, États-Unis, 11 Janvier 2014/15 Janvier 2014.

Résumé : Structural heterozygosity is thought to be one of the main causes of sterility in banana (Musa spp.) hybrids. These structural variations impact chromosomal segregation and recombination, limiting crossing possibilities and complicating genetic analyses. It is thus important to better understand their nature and location in the genome. For the production of the Musa accuminata reference genome sequence (D'Hont et al., Nature, 2012), we generated a genetic map that allowed anchoring 70% of the genome assembly on the 11 Musa chromosomes. The genetic map was based on a self progeny of the wild diploid Musa acuminata 'Pahang', the parent of the sequenced doubled haploid DH-Pahang. Strongly distorted markers were found on linkage group 1 and part of linkage group 4 that might indicate a structural rearrangement affecting these two linkage groups. We have developed a bioinformatic pipeline to help characterize structural variation based on resequencing approaches. We are using this pipeline on resequencing data of DH-Pahang and its parent and are densifying the genetic map with DArTseq markers to better understand the causes of the strong markers distortions and their link with structural variation.

Classification Agris : F30 - Génétique et amélioration des plantes
F63 - Physiologie végétale - Reproduction

Auteurs et affiliations

• Martin Guillaume, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1801-7500
• Carreel Françoise, CIRAD-BIOS-UMR AGAP (FRA)
• Baurens Franc-Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5219-8771
• Cardi Céline, CIRAD-BIOS-UMR AGAP (FRA)
• Kilian Andrzej, Diversity Arrays Technology (AUS)
• Rouard Mathieu, Bioversity International (FRA)
• Jenny Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6165-6888
• D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/573279/)

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