Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): Results from an international ring trial targeting four species of the subgenus Avaritia

Garros Claire, Balenghien Thomas, Carpenter Simon, Delecolle Jean Claude, Meiswinkel Rudolf, Pedarrieu Aurélie, Rakotoarivony Ignace, Gardes Laëtitia, Golding Nick, Barber James, Miranda Miguel, Borràs Borràs David, Goffredo Maria, Monaco Federica, Pagès Nonito, Sghaier Soufien, Hammami Salah, Calvo Jorge H., Lucientes Javier, Geysen Dirk, De Deken Gill, Sarto i Monteys Victor, Schwenkenbeche Jan, Kampen Helge, Hoffmann Bernd, Lehmann Kathrin, Werner Doreen, Baldet Thierry, Lancelot Renaud, Cetre-Sossah Catherine. 2014. Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): Results from an international ring trial targeting four species of the subgenus Avaritia. Parasites and Vectors, 7 (223), 9 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
Use under authorization by the author or CIRAD.

Télécharger (213kB) | Preview

Quartile : Q1, Sujet : PARASITOLOGY

Abstract : Background Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are biological vectors of internationally important arboviruses. To understand the role of Culicoides in the transmission of these viruses, it is essential to correctly identify the species involved. Within the western Palaearctic region, the main suspected vector species, C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus, have similar wing patterns, which makes it difficult to separate and identify them correctly. Methods In this study, designed as an inter-laboratory ring trial with twelve partners from Europe and North Africa, we assess four PCR-based assays which are used routinely to differentiate the four species of Culicoides listed above. The assays based on mitochondrial or ribosomal DNA or microarray hybridisation were tested using aliquots of Culicoides DNA (extracted using commercial kits), crude lysates of ground specimens and whole Culicoides (265 individuals), and non-Culicoides Ceratopogonidae (13 individuals) collected from across Europe. Results A total of 800 molecular assays were implemented. The in-house assays functioned effectively, although specificity and sensitivity varied according to the molecular marker and DNA extraction method used. The Obsoletus group specificity was overall high (95-99%) while the sensitivity varied greatly (59.6-100%). DNA extraction methods impacted the sensitivity of the assays as well as the type of sample used as template for the DNA extraction. Conclusions The results are discussed in terms of current use of species diagnostic assays and the future development of molecular tools for the rapid differentiation of cryptic Culicoides species. (Résumé d'auteur)

Mots-clés Agrovoc : Culicoides, Identification, PCR, Biologie moléculaire

Mots-clés géographiques Agrovoc : Afrique du Nord, Europe

Mots-clés complémentaires : Culicoides obseletus, Avaritia

Classification Agris : L72 - Pests of animals
U30 - Research methods

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Garros Claire, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0003-4378-5031
  • Balenghien Thomas, CIRAD-BIOS-UMR CMAEE (FRA)
  • Carpenter Simon, The Pirbright Institute (GBR)
  • Delecolle Jean Claude, Faculté de médecine de Strasbourg (FRA)
  • Meiswinkel Rudolf, Central Veterinary Institute (NLD)
  • Pedarrieu Aurélie, CIRAD-BIOS-UMR CMAEE (FRA)
  • Rakotoarivony Ignace, CIRAD-BIOS-UMR CMAEE (FRA)
  • Gardes Laëtitia, CIRAD-BIOS-UMR CMAEE (FRA)
  • Golding Nick, The Pirbright Institute (GBR)
  • Barber James, The Pirbright Institute (GBR)
  • Miranda Miguel, UIB (ESP)
  • Borràs Borràs David, UIB (ESP)
  • Goffredo Maria, IZSA&M (ITA)
  • Monaco Federica, IZSA&M (ITA)
  • Pagès Nonito, UAB (ESP) ORCID: 0000-0001-6020-7175
  • Sghaier Soufien, IRVT (TUN)
  • Hammami Salah, IRVT (TUN)
  • Calvo Jorge H., CITA [Centro de Investigacion y Tecnologia Agroalimentaria] (ESP)
  • Lucientes Javier, Universidad de Zaragoza (ESP)
  • Geysen Dirk, Institute of Tropical Medicine (BEL)
  • De Deken Gill, Institute of Tropical Medicine (BEL)
  • Sarto i Monteys Victor, ICTA [Espagne] (ESP)
  • Schwenkenbeche Jan, University of Aberdeen (GBR)
  • Kampen Helge, Federal Research Institute for Animal Health (DEU)
  • Hoffmann Bernd, FLI (DEU)
  • Lehmann Kathrin, Humboldt Universität zu Berlin (DEU)
  • Werner Doreen, Institut für Landnutzungssysteme (DEU)
  • Baldet Thierry, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0003-2979-9517
  • Lancelot Renaud, CIRAD-BIOS-UMR CMAEE (FRA)
  • Cetre-Sossah Catherine, CIRAD-BIOS-UMR CMAEE (REU)

Source : Cirad - Agritrop (

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-06-08 ]