Updates on the Musa acuminata reference genome sequence [W074]

D'Hont Angélique, Martin Guillaume, Baurens Franc-Christophe, Droc Gaëtan, Cenci Alberto, Guignon Valentin, Ruas Max, Carreel Françoise, Kilian Andrzej, Aury Jean-Marc, Hastie Alex, Dolezel Jaroslav, Roux Nicolas, Bocs Stéphanie, Rouard Mathieu. 2015. Updates on the Musa acuminata reference genome sequence [W074]. In : Plant and Animal Genomes Conference XXIII Conference, San diego, United States, San Diego, United States, January 10-14, 2015. s.l. : s.n., Résumé Plant and Animal Genome Conference. 23, San Diego, États-Unis, 10 Janvier 2015/14 Janvier 2015.

Résumé : Since the release of the Banana (Musa acuminataDH Pahang) sequence in July 2012, significant progresses were made to improve the quality of this reference sequence as well as the access to this information. This presentation intends to give an update on the status of the sequence assembly and gene annotation as well as the information systems that handle those data. An improved version of the assembly was produced (Martin et al in Prep.). The total scaffold number was reduced of 79% (7513 vs 1532), with a N50 that increased from 1.3Mb (65 scaffolds) to 3.0 Mb (26 scaffolds). Unknown sites (N) were reduced from 17.3% to 10.0%. Finally 89.5% of assembly was anchored to the 11 chromosomes compared to the previous 70%. The unanchored sequences represent now 46.5 Mb compare to 140.4 Mb before. For the gene annotation, a new release of annotation is in progress that have been mapped on the assembly v2 and will take advantage of improvements coming from manual curation (~ 2,000 genes out of the 36,542) and NCBI RefSeq released in October 2014. Finally, an overview of the new version of the Banana Genome Hub (Droc et al, 2013) will be presented. It includes additional features like JBrowse, a chromosome Browser and is being linked to the resources genetics being genotyped by Next Generation Genotyping and accessible via the Musa Germplasm Information System (MGIS). (Texte intégral)

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)
• Martin Guillaume, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1801-7500
• Baurens Franc-Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5219-8771
• Droc Gaëtan, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-1849-1269
• Cenci Alberto, Bioversity International (ITA)
• Guignon Valentin, Bioversity International (FRA)
• Ruas Max, Bioversity International (FRA)
• Carreel Françoise, CIRAD-BIOS-UMR AGAP (FRA)
• Kilian Andrzej, Diversity Arrays Technology (AUS)
• Aury Jean-Marc, CEA (FRA)
• Hastie Alex, BioNano Genomics (USA)
• Dolezel Jaroslav, Institute of Experimental Botany (CZE)
• Roux Nicolas, Bioversity International (FRA)
• Bocs Stéphanie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-7850-4426
• Rouard Mathieu, Bioversity International (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/575377/)

Métriques

[ Page générée et mise en cache le 2022-07-18 ]