Evaluation of thermal stability and inactivation of local Peste des Petits Ruminants isolates cultivated in cell culture

Latif Asma, Libeau Geneviève, Zahur Aamer Bin, Zahra Rabab, Afzal Muhammad. 2016. Evaluation of thermal stability and inactivation of local Peste des Petits Ruminants isolates cultivated in cell culture. In : Actes du Printemps de Baillarguet. 8ème édition. Berthelot Edwige (ed.), Diagne Christophe (ed.), Hammami Pachka (ed.), Lesieur Vincent (ed.), Lies Adrien (ed.), Rombaut Antoine (ed.). INRA, EBCL, Ecole Doctorale GAIA, CeMEB, CIRAD, IRD, Montpellier SupAgro. Montferrier : INRA-CIRAD, Résumé, 41. Printemps de Baillarguet. 8, Montferrier-sur-Lez, France, 2 Juin 2016/3 Juin 2016.

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Résumé : Peste des Petits Ruminants is a very contagious viral disease affecting large number of small ruminants predominantly goats and sheep. Vaccines are available for controlling this fatal disease but the efficacy of these vaccines depends upon the maintenance of cold chain in areas of extreme weather conditions. Uptil now, little is known about the stability of PPRV and its inactivation at different environmental conditions. In the current study, thermal stability of seven cell culture derived PPRV isolates under different environmental temperatures and the ability of heat and UVC irradiation to inactivate PPRV were evaluated. To assess the thermal stability, aliquots of PPRV isolates incubated at 37_C were removed after every three hours while those incubated at RT and 4_C were removed every day and every two days respectively. To evaluate the sensitivity of extreme heat, aliquots were subjected to four increasing temperatures (56_C, 60_C, 62_C and 65_C) for different time intervals. To investigate the effects of UVC on PPRV infectivity, aliquots were subjected to UVC irradiation for three different time points. Tissue culture infective dose (TCID50) of all the treated samples was calculated to determine the infectious titers using Vero cells. The results of the current study reveals that thermal stability of PPRV may be influenced by high temperatures, extreme heat and UVC irradiation and selecting the suitable heat resistant PPRV isolates may be a good approach to develop a thermostable vaccine for PPR.

Mots-clés libres : PPR, Thermal stability, Cell culture

Classification Agris : L73 - Maladies des animaux

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