Velogenic Newcastle disease virus takes advantage of transmission capabilities to suppress lentogenic virus during coinfection

Liu Haijin, Servan de Almeida Renata, Gil Patricia, Albina Emmanuel. 2016. Velogenic Newcastle disease virus takes advantage of transmission capabilities to suppress lentogenic virus during coinfection. In : Programme and abstracts EPIZONE Going Viral. EPIZONE, INIA. Madrid : EPIZONE, Résumé, 43. Annual Meeting EPIZONE Going Viral. 10, Madrid, Espagne, 27 Septembre 2016/29 Septembre 2016.

Résumé : Since the first Newcastle disease virus (NDV) isolated in 1920s, many other strains have been successfully isolated. Most of these strains are velogenic or at least having a virulent-like fusion protein's cleavage sites. Newcastle disease vaccines were developed several decades ago and since have been used extensively worldwide. This continued vaccination with “old” vaccine strains is thought to drive viral evolution and foster the emergence of virulent strains. Another possibility is the better fitness of virulent strains for transmission and amplification in the targets species that makes lentogenic strains less frequent in the field. In order to test whether the virulent NDV strains take advantages of their transmission capabilities to suppress the nonvirulent strains, reverse genetics was used to generate one velogenic and one lentogenic NDV strains with the same genetic background and with EGFP and RFP markers, respectively. These viruses were used to individually infect or co-infect BHK-21 cells. Both strains could persistently infect the cells and showed similar ability of propagation over 15-16 cell passages. However, when co-infected, the velogenic strain took control on the lentogenic strain that gradually disappeared until the 10thpassage. This competition could partially explain why most isolated NDV strains are velogenic. (Texte intégral)

Mots-clés libres : Newcastle disease virus, Transmission, Competition

Classification Agris : L73 - Maladies des animaux

Auteurs et affiliations

• Liu Haijin, CIRAD-BIOS-UMR CMAEE (FRA)
• Servan de Almeida Renata, CIRAD-BIOS-UMR CMAEE (FRA)
• Gil Patricia, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0001-7984-7340
• Albina Emmanuel, CIRAD-BIOS-UMR CMAEE (GLP)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/582766/)

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