Evolution of the banana genome is impacted by large chromosomal translocations

Martin Guillaume, Carreel Françoise, Coriton Olivier, Hervouet Catherine, Cardi Céline, Derouault Paco, Roques Danièle, Salmon Frédéric, Rouard Mathieu, Sardos Julie, Labadie Karine, Baurens Franc-Christophe, D'Hont Angélique. 2017. Evolution of the banana genome is impacted by large chromosomal translocations. . Montpellier : INRA, Résumé, 1 p. Workshop DynaGeV : Dynamique des Génomes Végétaux. 3, Montpellier, France, 8 Juin 2017/9 Juin 2017.

Résumé : Most banana cultivars are triploid derived from Musa acuminata (2n=2x=22), sometimes combined with Musa balbisiana (2n=2x=22). These species and subspecies diverged following geographical isolation in distinct Southeast Asian continental regions and islands. Contact between them was made possible by human migration and led to the selection of seedless parthenocarpic hybrids. M. acuminata subspecies were suggested to differ by a few large chromosomal rearrangements based on chromosome pairing configurations in inter-subspecies hybrids. We searched for large chromosomal rearrangements in a seedy M. acuminata ssp. malaccensis banana accession through mate-pair sequencing, BAC-FISH, targeted PCR and marker (DArTseq) segregation in its progeny. We identified a heterozygous reciprocal translocation involving two distal 3 Mb and 10 Mb segments from chromosomes 01 and 04, respectively, and showed that it locally generated high segregation distortions and reduced recombinations in its progeny. The two chromosome structures were found to be mutually exclusive in gametes and the rearranged structure was preferentially transmitted to the progeny. The rearranged chromosome structure was frequently found in triploid cultivars but within the wild accessions, it was only found within malaccensis sub-species accessions, thus suggesting that this rearrangement occurred in this sub-species. We propose mechanisms for the spread of this rearrangement in Musa diversity and propose that this structure may have played a role in the emergence of triploid cultivars. (Texte intégral)

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Martin Guillaume, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1801-7500
• Carreel Françoise, CIRAD-BIOS-UMR AGAP (FRA)
• Coriton Olivier, INRA (FRA)
• Hervouet Catherine, CIRAD-BIOS-UMR AGAP (FRA)
• Cardi Céline, CIRAD-BIOS-UMR AGAP (FRA)
• Derouault Paco
• Roques Danièle, CIRAD-BIOS-UMR AGAP (GLP)
• Salmon Frédéric, CIRAD-BIOS-UMR AGAP (GLP)
• Rouard Mathieu, Bioversity International (FRA)
• Sardos Julie, Bioversity International (FRA)
• Labadie Karine, Institut de génomique (FRA)
• Baurens Franc-Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5219-8771
• D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/584604/)

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