Study of the interactions of a major rubber particle protein (REF1) with synthetic phospholipids in Langmuir monolayers

Wadeesirisak Kanthida, Castano Sabine, Caudy Nadia, Vaysse Laurent, Peruch Frédéric, Rattanaporn Kittipong, Liengprayoon Siriluck, Roytrakul Sittiruk, Lecomte Sophie, Bottier Céline. 2017. Study of the interactions of a major rubber particle protein (REF1) with synthetic phospholipids in Langmuir monolayers. . IRRDB. Jakarta : IRRDB, 10 p. International Rubber Conference & IRRDB Annual Meeting 2017, Jakarta, Indonésie, 18 Octobre 2017/20 Octobre 2017.

Résumé : Rubber Elongation Factor (REF1) protein is located on the surface of rubber particles (RPs). As a RP-bound and hydrophobic protein, REF1 is suspected to be only partially eliminated by the water washing occurring during latex processing into raw natural rubber (NR) in the form of unsmoked rubber sheet (USS) and could thus affect raw NR properties. In this work, REF1 was identified as an abundant protein of raw NR and bio physical methods were used to describe the organization/structure of REF1 at the surface of RPs interacting with the lipid monolayer surrounding the poly-isoprene core. Therefore, an approach in Langmuir film was implemented to investigate the interactions between recombinant REF1 and synthetic phospholipids (POPC, POPG and POPA) by fluorescence (calcein-leakage measurements), ellipsometry, Brewster angle microscopy, and PM-IRRAS spectroscopy. REF1 was shown to interact differently depending on lipid headgroup type: no interaction with POPC, intermediate interactions with POPG and strong interactions associated to a conformational switch from α-helices to β-sheets with POPA.

Classification Agris : Q60 - Traitement des produits agricoles non alimentaires

Auteurs et affiliations

• Wadeesirisak Kanthida, CIRAD-PERSYST-UMR IATE (FRA)
• Castano Sabine, CNRS (FRA)
• Caudy Nadia, CNRS (FRA)
• Vaysse Laurent, CIRAD-PERSYST-UMR IATE (THA)
• Peruch Frédéric, CNRS (FRA)
• Rattanaporn Kittipong, KURDI (THA)
• Liengprayoon Siriluck, Kasetsart University (THA)
• Roytrakul Sittiruk, BIOTEC [National Center for Genetic Engineering and Biotechnology] (THA)
• Lecomte Sophie, CNRS (FRA)
• Bottier Céline, CIRAD-PERSYST-UMR IATE (THA) ORCID: 0000-0002-5318-4275

Source : Cirad-Agritrop (https://agritrop.cirad.fr/586134/)

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