Unleashing meiotic recombinaton in plants. [W1068]

Mieulet Delphine, Fernandes Joiselle B., Seguela-Arnaud Mathilde, Aubert Grégoire, Brès C., Guiderdoni Emmanuel, Mercier Raphael. 2018. Unleashing meiotic recombinaton in plants. [W1068]. In : Proceedings Plant and Animal Genome XXVI Conference. PAG. San Diego : PAG, Résumé, 1 p. Plant and Animal Genomes Conference. 26, San Diego, États-Unis, 13 Janvier 2018/17 Janvier 2018.

Résumé : Meiotic crossovers shuffle parental genetic information, providing novel combinations of alleles on which natural or artificial selection can act. However, crossover events are relatively rare, typically one to three exchange points per chromosome pair. Recent work has identified three pathways limiting meiotic crossovers in Arabidopsis thaliana that rely on the activity of FANCM [Crismani W, et al. (2012) Science 336:1588-1590], RECQ4 [Séguéla-Arnaud M, et al. (2015) Proc Natl Acad Sci USA 112:4713-4718], and FIGL1 [Girard C, et al. (2015) PLoS Genet 11:e1005369]. We analyzed recombination in Arabidopsis plants in which one, two, or all three of these pathways were disrupted in both pure line and hybrid contexts. The greatest effect was observed when combining recq4 and figl1 mutations, which increased the hybrid genetic map length from 389 to 3,037 cM. This corresponds to an unprecedented 7.8-fold increase in crossover frequency. We then showed that recombination can be massively increased in crop species. These results open the possibility of manipulating recombination to enhance plant breeding efficiency.

Classification Agris : F30 - Génétique et amélioration des plantes
F63 - Physiologie végétale - Reproduction

Auteurs et affiliations

• Mieulet Delphine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6220-0372
• Fernandes Joiselle B., INRA (FRA)
• Seguela-Arnaud Mathilde, INRA (FRA)
• Aubert Grégoire, INRA (FRA)
• Brès C.
• Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
• Mercier Raphael, Institut Jean-Pierre Bourgin (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587270/)

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