The intra-cellular localization of the vanillin biosynthetic machinery in pods of Vanilla planifolia

Gallage Nethaji J., Jorgensen Kirsten, Janfelt Christian, Nielsen Agnieszka J.Z., Naake Thomas, Dunski Eryk, Dalsten Lene, Grisoni Michel, Moller Birger Lindberg. 2017. The intra-cellular localization of the vanillin biosynthetic machinery in pods of Vanilla planifolia. Plant and Cell Physiology, 59 (2) : 304-318.

https://doi.org/10.1093/pcp/pcx185

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q2, Sujet : CELL BIOLOGY

Résumé : Vanillin is the most important flavor compound in the vanilla pod. Vanilla planifolia vanillin synthase (VpVAN) catalyzes the conversion of ferulic acid and ferulic acid glucoside into vanillin and vanillin glucoside, respectively. Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) of vanilla pod sections demonstrates that vanillin glucoside is preferentially localized within the mesocarp and placental laminae whereas vanillin is preferentially localized within the mesocarp. VpVAN is present as the mature form (25 kDa) but, depending on the tissue and isolation procedure, small amounts of the immature unprocessed form (40 kDa) and putative oligomers (50, 75 and 100 kDa) may be observed by immunoblotting using an antibody specific to the C-terminal sequence of VpVAN. The VpVAN protein is localized within chloroplasts and re-differentiated chloroplasts termed phenyloplasts, as monitored during the process of pod development. Isolated chloroplasts were shown to convert [14C]phenylalanine and [14C]cinnamic acid into [14C]vanillin glucoside, indicating that the entire vanillin de novo biosynthetic machinery converting phenylalanine to vanillin glucoside is present in the chloroplast.

Mots-clés géographiques Agrovoc : La Réunion, France

Classification Agris : F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

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