Improving quality control of contagious caprine pleuropneumonia vaccine with tandem mass spectrometry

Thiaucourt François, Pible Olivier, Miotello Guylaine, Nwankpa Nick, Armengaud Jean. 2018. Improving quality control of contagious caprine pleuropneumonia vaccine with tandem mass spectrometry. Proteomics, 18 (17):1800088, 5 p.

Quartile : Q2, Sujet : BIOCHEMICAL RESEARCH METHODS / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Vaccines to protect livestock against contagious caprine pleuropneumonia (CCPP) consist of inactivated, adjuvanted antigens. Quality control of these vaccines is challenging as total protein quantification provides no indication of protein identity or purity, and culture is not an option. Here, a tandem mass spectrometry approach is used to identify the mycoplasma antigen contained in reference samples and in commercial CCPP vaccines. By the same approach, the relative amounts of mycoplasma antigen and residual proteins originating from the production medium are determined. Mass spectrometry allows easy and rapid identification of the peptides present in the vaccine samples. Alongside the most probable mycoplasma species effectively present in the vaccines, a very high proportion of peptides from medium constituents are detected in the commercial vaccines tested.

Mots-clés géographiques Agrovoc : Afrique, Asie

Mots-clés libres : CCPP, Vaccine, Quality control

Classification Agris : L73 - Animal diseases
L70 - Veterinary science and hygiene - General aspects

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Thiaucourt François, CIRAD-BIOS-UMR ASTRE (FRA) - auteur correspondant
• Pible Olivier, CEA (FRA)
• Miotello Guylaine, CEA (FRA)
• Nwankpa Nick, Pan African Veterinary Vaccine Centre (ETH)
• Armengaud Jean, CEA (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/588807/)

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