Another night at the museum: searching for traces of plant viruses using the virion-associated nucleic acid metagenomics approach. [P.21]

Julian Charlotte, Filloux Denis, Rieux Adrien, Lefeuvre Pierre, Lett Jean-Michel, Becker Nathalie, Gagnevin Lionel, Trinder-Smith Terry H., Hartnady Penelope, Martin Darren Patrick, Roumagnac Philippe. 2019. Another night at the museum: searching for traces of plant viruses using the virion-associated nucleic acid metagenomics approach. [P.21]. In : Livre des résumés des 17 ème Rencontres de virologie végétale. INRA, CIRAD, CNRS, SFP, SFV. Aussois : INRA, Résumé, 80. Rencontres de Virologie Végétale (RVV 2019). 17, Aussois, France, 27 Janvier 2019/31 Janvier 2019.

Résumé : The overall purpose of this project was to retrieve plant virus sequences from preserved Mediterranean and tropical plant specimens currently held within European, American and African herbaria and use these to provide a temporal element to our viral diversity studies and to retrace the emergence and spread over the past 100 years of several Mediterranean and tropical plant virus species. More than 550 plant specimens were processed using the virion-associated nucleic acid (VANA) metagenomics-based approach. Illumina reads were cleaned up and de novo assembled. Several plant virus-related contigs were identified, including potyvirus-, alfamovirus-, luteovirus-, and foveavirus-related sequences. Interestingly, 26 contigs (31-362 nt in length) sharing high identity (83.9-100%) with Euphorbia ringspot virus were recovered from two Euphorbia caput-medusae specimens collected in South Africa in 1946 (22 contigs) and 1991 (4 contigs) and conserved in the Bolus Herbarium of Cape Town. The characterization of partial or complete genomes of these “ancient” potyvirus isolates is underway.

Auteurs et affiliations

• Julian Charlotte, CIRAD-BIOS-UMR BGPI (FRA)
• Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
• Rieux Adrien, CIRAD-BIOS-UMR PVBMT (REU)
• Lefeuvre Pierre, CIRAD-BIOS-UMR PVBMT (REU)
• Lett Jean-Michel, CIRAD-BIOS-UMR PVBMT (REU)
• Becker Nathalie, CIRAD-BIOS-UMR PVBMT (REU)
• Gagnevin Lionel, CIRAD-BIOS-UMR IPME (FRA) ORCID: 0000-0002-2943-0827
• Trinder-Smith Terry H., UCT (ZAF)
• Hartnady Penelope, CIRAD-BIOS-UMR BGPI (FRA)
• Martin Darren Patrick, UCT (ZAF)
• Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591004/)

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