Evaluation of Capripoxvirus surface proteins as antigens for the detection of antibodies using indirect ELISA

Cattoli Giovanni, Berguido Francisco, Gelayas Esayas, Krstevski Kiril, Loitsch Angelika, Comtet Loïc, Pourqier Philippe, Tuppurainen Eeva, Diallo Adama, Euloge Lamien Charles. 2018. Evaluation of Capripoxvirus surface proteins as antigens for the detection of antibodies using indirect ELISA. In : Book of abstracts of the ESVV 2018. ESVV. Vienne : ESVV, Résumé, 56. International Congress for Veterinary (ESVV 2018). 11, Vienne, Autriche, 27 Août 2018/30 Août 2018.

Note générale : A l'occasion de ce congrès, s'est également déroulé le 12. Annual Meeting of EPIZONE.

Résumé : Background Lumpy Skin Disease (LSD), Sheeppox (SPP) and Goatpox (GTP) are contagious diseases of ruminants with a devastating impact on the livestock industry and trade, also affecting the living conditions of poor rural and small farmers. LSD, SPP, GTP were mainly confined to Africa, the Middle East and Asia, with some sporadic incursions of SPP in Greece and Bulgaria. However, in 2015 the first incursions of LSD occurred in the European Union. Due to their potential for rapid spreading, a highly sensitive and specific serological method for active and passive surveillance of SPP, GTP and LSD is needed. In addition, such a tool could serve for post-vaccination monitoring. Objective The aim of this work was to evaluate recombinants proteins of the capripoxvirus virion for use in an indirect ELISA (iELISA) to detect anti-capripox antibodies in vaccinated and naturally infected small ruminants and cattle sera. Method We have identified, characterized, expressed and purified capripox-virus virion surface protein (CVSP) that react to positive SPP, GTP and LSD sera samples by Western Blot and iELISA. The iELISA was further optimised and evaluated using sera samples from vaccinat-ed, experimentally and naturally infected sheep, goat and cattle. Results Twenty experimentally infected positive and 130 negative LSD, SPP and GTP sera samples were tested and correctly identified using the CVSP iELISA. We tested sera collected at multiple time points from several LSD experimentally infected animals. We observed a time dependant increased in anti-LSD antibody production after day 14 post infection. For specificity, five ORF positive sera were tested. None of the ORF positive sera was positive in the CVSP iELISA. A number of field sera collected during capripoxvirus outbreaks and vaccination campaigns from animals with known infection/vaccina-tion status were tested by the CVSP iELISA and compared to the virus neutralization assay. The complete dataset and results of the field test evaluation will be presented. Conclusion As capripox diseases spread, and to strengthen disease surveillance and control programmes, there is an ever-increasing need for rapid and effective antibody detection assays. The CVSP tested in the pres-ent study demonstrated to be good antigen candidates for the de-velopment of sensitive and specific serological assays. The prototype iELISA developed has the potential to be used as an effective and rapid method for capripoxvirus antibody detection in vaccinated, experimentally and naturally infected sheep, goat and cattle sera.

Auteurs et affiliations

• Cattoli Giovanni, IAEA (AUT)
• Berguido Francisco, FAO (AUT)
• Gelayas Esayas, NVI (ETH)
• Krstevski Kiril, University of Macedonia (GRC)
• Loitsch Angelika, Austrian Agency for Health and Food Safety (AUT)
• Comtet Loïc, IDvet (FRA)
• Pourqier Philippe, IDvet (FRA)
• Tuppurainen Eeva, Institute of Animal Health (GBR)
• Diallo Adama, CIRAD-BIOS-UMR ASTRE (SEN)
• Euloge Lamien Charles, IAEA (AUT)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591057/)

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