Plasmodium falciparum parasite population structure and gene flow associated to anti-malarial drugs resistance in Cambodia

Dwivedi Ankit, Khim Nimol, Reynes Christelle, Ravel Patrice, Ma Laurence, Tichit Magali, Bourchier Christiane, Kim Saorin, Dourng Dany, Khean Chanra, Chim Pheaktra, Siv Sovannaroth, Frutos Roger, Lek Dysoley, Mercereau-Puijalon Odile, Ariey Frédéric, Menard Didier, Cornillot Emmanuel. 2016. Plasmodium falciparum parasite population structure and gene flow associated to anti-malarial drugs resistance in Cambodia. Malaria Journal, 15:319, 12 p.

Quartile : Q1, Sujet : TROPICAL MEDICINE / Quartile : Q2, Sujet : PARASITOLOGY / Quartile : Q2, Sujet : INFECTIOUS DISEASES

Résumé : Background: Western Cambodia is recognized as the epicentre of emergence of Plasmodium falciparum multi-drug resistance. The emergence of artemisinin resistance has been observed in this area since 2008–2009 and molecular signatures associated to artemisinin resistance have been characterized in k13 gene. At present, one of the major threats faced, is the possible spread of Asian artemisinin resistant parasites over the world threatening millions of people and jeopardizing malaria elimination programme efforts. To anticipate the diffusion of artemisinin resistance, the identification of the P. falciparum population structure and the gene flow among the parasite population in Cambodia are essential. Methods: To this end, a mid-throughput PCR-LDR-FMA approach based on LUMINEX technology was developed to screen for genetic barcode in 533 blood samples collected in 2010–2011 from 16 health centres in malaria endemics areas in Cambodia. Results: Based on successful typing of 282 samples, subpopulations were characterized along the borders of the country. Each 11-loci barcode provides evidence supporting allele distribution gradient related to subpopulations and gene flow. The 11-loci barcode successfully identifies recently emerging parasite subpopulations in western Cambodia that are associated with the C580Y dominant allele for artemisinin resistance in k13 gene. A subpopulation was identified in northern Cambodia that was associated to artemisinin (R539T resistant allele of k13 gene) and mefloquine resistance. Conclusions: The gene flow between these subpopulations might have driven the spread of artemisinin resistance over Cambodia.

Mots-clés Agrovoc : Plasmodium falciparum, résistance aux médicaments, artémisinine, malaria, parasite, flux de gènes

Mots-clés géographiques Agrovoc : Cambodge

Mots-clés complémentaires : méfloquine

Classification Agris : S50 - Santé humaine
L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Dwivedi Ankit, Institut de Biologie Computationnelle (FRA)
• Khim Nimol, Institut Pasteur du Cambodge (KHM)
• Reynes Christelle, UM1 (FRA)
• Ravel Patrice, Institut de Recherche en Cancérologie de Montpellier (FRA)
• Ma Laurence, Institut Pasteur (FRA)
• Tichit Magali, Institut Pasteur (FRA)
• Bourchier Christiane, Institut Pasteur (FRA)
• Kim Saorin, Institut Pasteur du Cambodge (KHM)
• Dourng Dany, Institut Pasteur du Cambodge (KHM)
• Khean Chanra, Institut Pasteur du Cambodge (KHM)
• Chim Pheaktra, Institut Pasteur du Cambodge (KHM)
• Siv Sovannaroth, National Centre for Parasitology, Entomology and Malaria Control (KHM)
• Frutos Roger, CIRAD-BIOS-UMR INTERTRYP (FRA)
• Lek Dysoley, National Centre for Parasitology, Entomology and Malaria Control (KHM)
• Mercereau-Puijalon Odile, Institut Pasteur (FRA)
• Ariey Frédéric, Institut Pasteur (FRA)
• Menard Didier, Institut Pasteur du Cambodge (KHM)
• Cornillot Emmanuel, Institut de Biologie Computationnelle (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591883/)

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