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Differences in microbiota between two multilocus lineages of the sugarcane Aphid (Melanaphis sacchari) in the continental United States

Holt Jocelyn R., Styer Alex, White Jennifer A., Armstrong John Scott, Nibouche Samuel, Costet Laurent, Malacrinò Antonino, Antwi Josephine B., Wulff Jason, Peterson Garry, McLaren Neal, Medina Raul F.. 2020. Differences in microbiota between two multilocus lineages of the sugarcane Aphid (Melanaphis sacchari) in the continental United States. Annals of the Entomological Society of America, 113 (4), n.spéc. Advanced Genetic Analysis of Invasive Arthropods:saaa003 : pp. 257-265.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Url - jeu de données : https://github.com/amalacrino/SCA_microbiota

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Additional Information : Raw data can be found on NCBI under accession number PRJNA578411. Scripts for data analysis are available through GitHub at: https://github.com/amalacrino/SCA_microbiota.

Abstract : The sugarcane aphid (SCA), Melanaphis Sacchari (Zehntner) (Hemiptera: Aphididae), has been considered an invasive pest of sugarcane in the continental United States since 1977. Then, in 2013, SCA abruptly became a serious pest of U.S. sorghum and is now a sorghum pest in 22 states across the continental United States. Changes in insect-associated microbial community composition are known to influence host-plant range in aphids. In this study, we assessed whether changes in microbiota composition may explain the SCA outbreak in U.S. sorghum. We characterized the SCA bacterial microbiota on sugarcane and grain sorghum in four U.S. states, using a metabarcoding approach. In addition, we used taxon-specific polymerase chain reaction (PCR) primers to screen for bacteria commonly reported in aphid species. As anticipated, all SCA harbored the primary aphid endosymbiont Buchnera aphidicola, an obligate mutualistic bacterial symbiont. Interestingly, none of the secondary symbionts, facultative bacteria typically associated with aphids (e.g., Arsenophonus, Hamiltonella, Regiella) were present in either the metabarcoding data or PCR screens (with the exception of Rickettsiella and Serratia, which were detected by metabarcoding at low abundances <1%). However, our metabarcoding detected bacteria not previously identified in aphids (Arcobacter, Bifidobacterium, Citrobacter). Lastly, we found microbial host-associated differentiation in aphids that seems to correspond to genetically distinct aphid lineages that prefer to feed on grain sorghum (MLL-F) versus sugarcane (MLL-D).

Mots-clés Agrovoc : Aphidoidea, Ravageur des plantes, Saccharum, Sorghum, Espèce envahissante, Flore microbienne

Mots-clés géographiques Agrovoc : États-Unis

Mots-clés complémentaires : Melanaphis sacchari, barcoding moleculaire

Mots-clés libres : Metabarcoding, Aphid, Invasive pest, Sugarcane, Sorghum, Microbiota

Classification Agris : H10 - Pests of plants
L10 - Animal genetics and breeding
L50 - Animal physiology and biochemistry

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Holt Jocelyn R., Texas A & M University (USA)
  • Styer Alex, University of Kentucky (USA)
  • White Jennifer A., University of Kentucky (USA)
  • Armstrong John Scott, USDA (USA)
  • Nibouche Samuel, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-2675-1382
  • Costet Laurent, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-3199-2885
  • Malacrinò Antonino, Ohio State University (USA)
  • Antwi Josephine B., University of Mary Washington (USA)
  • Wulff Jason, Texas A & M University (USA)
  • Peterson Garry, Texas A&M AgriLife Research (USA)
  • McLaren Neal, University of the Free State (ZAF)
  • Medina Raul F., Texas A & M University (USA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/595339/)

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