Optimization of a protein extraction method from natural rubber sheets made of Hevea brasiliensis latex

Srisomboon Surattiya, Wadeesirisak Kanthida, Vaysse Laurent, Sainte-Beuve Jérôme, Musigamart Natedao, Liengprayoon Siriluck, Bonfils Frédéric, Rattanaporn Kittipong, Bottier Céline. 2021. Optimization of a protein extraction method from natural rubber sheets made of Hevea brasiliensis latex. Journal of Rubber Research, 24 : 27-39.

Quartile : Q4, Sujet : POLYMER SCIENCE

Résumé : A method to extract proteins from natural rubber (NR) samples has been developed to characterize the protein composition of NR by SDS-PAGE electrophoresis. While the incubation of NR pieces in aqueous buffers did not provide satisfactory results (no protein bands detected by SDS-PAGE), the solubilization of NR pieces in an organic solvent, prior to centrifugation and protein extraction from the obtained pellet, was found efficient to highlight protein bands by SDS-PAGE. Pure cyclohexane was used to solubilize NR pieces and resulted in a low share of nitrogen in the pellet (29%). The addition of 10% (v/v) ethanol to cyclohexane allowed to significantly increase this share to 73% and to detect 13 protein bands by SDS-PAGE. The molecular weights of the four most intense bands suggest the presence in NR of the following proteins: rubber elongation factor, small rubber particle protein, hevamine, and β-1-3 glucanase.

Mots-clés Agrovoc : Hevea brasiliensis, extraction, caoutchouc, électrophorèse gel polyacrylamide, qualité protéique

Mots-clés complémentaires : méthode d'extraction

Mots-clés libres : Natural rubber, Hevea brasiliensis, Protein extraction, SDS-PAGE electrophoresis

Classification Agris : Q60 - Traitement des produits agricoles non alimentaires
U50 - Sciences physiques et chimie

Champ stratégique Cirad : CTS 7 (2019-) - Hors champs stratégiques

Auteurs et affiliations

• Srisomboon Surattiya, Kasetsart University (THA)
• Wadeesirisak Kanthida, Kasetsart University (THA)
• Vaysse Laurent, CIRAD-PERSYST-UMR IATE (THA)
• Sainte-Beuve Jérôme, CIRAD-PERSYST-UMR IATE (FRA)
• Musigamart Natedao, Kasetsart University (THA)
• Liengprayoon Siriluck, Kasetsart University (THA)
• Bonfils Frédéric, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0001-7016-4770
• Rattanaporn Kittipong, Kasetsart University (THA) - auteur correspondant
• Bottier Céline, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0002-5318-4275 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/597312/)

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