Photoperiod-dependent transcriptional modifications in key metabolic pathways in Coffea arabica

Djerrab Doaa, Bertrand Benoît, Breitler Jean-Christophe, Leran Sophie, Dechamp Eveline, Campa Claudine, Barrachina Célia, Conejero Geneviève, Etienne Hervé, Sulpice Ronan. 2021. Photoperiod-dependent transcriptional modifications in key metabolic pathways in Coffea arabica. Tree Physiology, 41 (2) : 302-316.

Quartile : Q1, Sujet : FORESTRY

Résumé : Photoperiod length induces in temperate plants major changes in growth rates, morphology and metabolism with, for example, modifications in the partitioning of photosynthates to avoid starvation at the end of long nights. However, this has never been studied for a tropical perennial species adapted to grow in a natural photoperiod close to 12 h/12 h all year long. We grew Coffea arabica L., an understorey perennial evergreen tropical species in its natural 12 h/12 h and in a short 8 h/16 h photoperiod, and we investigated its responses at the physiological, metabolic and transcriptomic levels. The expression pattern of rhythmic genes, including core clock genes, was affected by changes in photoperiod. Overall, we identified 2859 rhythmic genes, of which 89% were also rhythmic in Arabidopsis thaliana L. Under short-days, plant growth was reduced, and leaves were thinner with lower chlorophyll content. In addition, secondary metabolism was also affected with chlorogenic acid and epicatechin levels decreasing, and in agreement, the genes involved in lignin synthesis were overexpressed and those involved in the flavanol pathway were underexpressed. Our results show that the 8 h/16 h photoperiod induces drastic changes in morphology, metabolites and gene expression, and the responses for gene expression are similar to those observed in the temperate annual A. thaliana species. Short photoperiod induces drastic changes in gene expression, metabolites and leaf structure, some of these responses being similar to those observed in A. thaliana.

Mots-clés Agrovoc : Coffea arabica, Arabidopsis thaliana, rythme circadien, photopériodicité, expression des gènes, arn messager, transcriptome

Mots-clés libres : Coffee, Circadian clock, Photoperiod, Transcriptome analysis

Classification Agris : F60 - Physiologie et biochimie végétale
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement européennes : European Commission

Projets sur financement : (EU) BREEDing Coffee for AgroForestry Systems

Auteurs et affiliations

• Djerrab Doaa, CIRAD-BIOS-UMR IPME (FRA)
• Bertrand Benoît, CIRAD-BIOS-UMR IPME (FRA) ORCID: 0000-0003-1969-3479 - auteur correspondant
• Breitler Jean-Christophe, CIRAD-BIOS-UMR IPME (MEX)
• Leran Sophie, CIRAD-BIOS-UMR IPME (FRA)
• Dechamp Eveline, CIRAD-BIOS-UMR RPB (FRA)
• Campa Claudine, IRD (FRA)
• Barrachina Célia, CNRS (FRA)
• Conejero Geneviève, INRAE (FRA)
• Etienne Hervé, CIRAD-BIOS-UMR RPB (FRA) ORCID: 0000-0002-6208-9982
• Sulpice Ronan, University of Ireland (IRL)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/597444/)

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