Evaluation of fecal immunoassays for canine Echinococcus infection in China

Wang Liying, Wang Qian, Cai Huixia, Huang Yan, Feng Yu, Bai Xuefei, Qin Min, Manguin Sylvie, Gavotte Laurent, Wu Weiping, Frutos Roger. 2021. Evaluation of fecal immunoassays for canine Echinococcus infection in China. PLoS Neglected Tropical Diseases, 15 (3):e0008690, 11 p.

Quartile : Q1, Sujet : PARASITOLOGY / Quartile : Q1, Sujet : TROPICAL MEDICINE

Résumé : Human echinococcosis is present worldwide but it is in China that disease prevalence is the highest. In western China, especially in the Tibetan Plateau, the burden of echinococcosis is the most important. Dogs are a major definitive host of Echinococcus and monitoring the presence of Echinococcus worms in dogs is therefore essential to efficiently control the disease. Detection kits based on three different technologies including sandwich ELISA, (indirect) ELISA, and gold immunodiffusion, are currently marketed and used in China. The objective of this work was to assess the efficacy of these kits, in particular with respect to sensitivity and specificity. Four fecal antigen detection kits for canine infection reflecting the three technologies were obtained from companies and tested in parallel on 220 fecal samples. The results indicate that the performance is lower than expected, in particular in terms of sensitivity. The best results were obtained with the sandwich ELISA technology. The gold immunofiltration yielded the poorest results. In all cases, further development is needed to improve the performance of these kits which are key components for the control of echinococcosis.

Mots-clés Agrovoc : surveillance épidémiologique, santé animale, echinococcose, technique immunologique, Test ELISA, ELISA indirect, fèces, chien

Mots-clés géographiques Agrovoc : Chine

Classification Agris : L73 - Maladies des animaux
L70 - Sciences et hygiène vétérinaires - Considérations générales

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Wang Liying, Chinese Center for Disease Control and Prevention (CHN)
• Wang Qian, Sichuan Provincial Center for Disease Control and Prevention (CHN)
• Cai Huixia, Qinghai Provincial Institute for Endemic Disease Prevention and Control (CHN)
• Huang Yan, Sichuan Provincial Center for Disease Control and Prevention (CHN)
• Feng Yu, Gansu Provincial Center for Disease Control and Prevention (CHN)
• Bai Xuefei, Sichuan Provincial Center for Disease Control and Prevention (CHN)
• Qin Min, Chinese Center for Disease Control and Prevention (CHN)
• Manguin Sylvie, IRD (FRA)
• Gavotte Laurent, UM2 (FRA)
• Wu Weiping, Chinese Center for Disease Control and Prevention (CHN) - auteur correspondant
• Frutos Roger, CIRAD-BIOS-UMR INTERTRYP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/599486/)

Métriques

[ Page générée et mise en cache le 2024-01-29 ]