Once upon a time, the VANA story based on the review "virion-associated nucleic acid-based metagenomics : a decade of advances in molecular characterization of plant viruses"

Moubset Oumaima, François Sarah, Maclot François, Palanga Essowé, Julian Charlotte, Claude Lisa, Fernandez Emmanuel, Rott Philippe, Daugrois Jean-Heinrich, Antoine-Lorquin Aymeric, Bernardo Pauline, Blouin Arnaud G., Temple Coline, Kraberger Simona, Fontenele Rafaela S, Harkins Gordon William, Ma Yuxin, Marais Armelle, Candresse Thierry, Ben Chehida Sélim, Lefeuvre Pierre, Lett Jean-Michel, Varsani Arvind, Massart Sébastien, Ogliastro Mylène, Martin Darren Patrick, Filloux Denis, Roumagnac Philippe. 2023. Once upon a time, the VANA story based on the review "virion-associated nucleic acid-based metagenomics : a decade of advances in molecular characterization of plant viruses". In : 19e Rencontres de virologie végétale (RVV2023). Résumés. SFV, SFP, RIVOC, IRD, INRAE, CNRS, CIRAD, Agropolis. Aussois : SFV, Résumé, p. 91. Rencontres de virologie végétale (RVV2023). 19, Aussois, France, 15 Janvier 2023/19 Janvier 2023.

Résumé : Over the last decade, viral metagenomic studies have resulted in the discovery of thousands of previously unknown viruses. These studies are likely to play a pivotal role in obtaining an accurate and robust understanding of how viruses affect the stability and productivity of ecosystems. Among the metagenomics-based approaches that have been developed since the beginning of the 21st century, shotgun metagenomics applied specifically to virion-associated nucleic acids (VANA) has been used to disentangle the diversity of the viral world. We summarize herein the results of 25 VANA-based studies, focusing on plant and insect samples conducted over the last decade (2010 to 2022). Collectively, viruses from 94 different families were reliably detected in these studies, including capsidless RNA viruses that replicate in fungi, oomycetes, and plants. Finally, strengths and weaknesses of the VANA approach are summarized and perspectives of applications in detection, epidemiological surveillance, environmental monitoring, and ecology of plant viruses are provided.

Auteurs et affiliations

• Moubset Oumaima, CIRAD-BIOS-UMR PHIM (FRA)
• François Sarah, University of Oxford (GBR)
• Maclot François, GxABT (BEL)
• Palanga Essowé, ITRA (TGO)
• Julian Charlotte, CIRAD-BIOS-UMR PHIM (FRA)
• Claude Lisa, Université de Montpellier (FRA)
• Fernandez Emmanuel, CIRAD-BIOS-UMR PHIM (FRA)
• Rott Philippe, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0001-6085-6159
• Daugrois Jean-Heinrich, CIRAD-BIOS-UMR PHIM (FRA)
• Antoine-Lorquin Aymeric, Université de Montpellier (FRA)
• Bernardo Pauline, Enza Zaden (NLD)
• Blouin Arnaud G., GxABT (BEL)
• Temple Coline, GxABT (BEL)
• Kraberger Simona, Arizona State University (USA)
• Fontenele Rafaela S, Arizona State University (USA)
• Harkins Gordon William, University of the Western Cape (ZAF)
• Ma Yuxin, Université de Bordeaux (FRA)
• Marais Armelle, INRAE (FRA)
• Candresse Thierry, INRAE (FRA)
• Ben Chehida Sélim, CIRAD-BIOS-UMR PVBMT (REU)
• Lefeuvre Pierre, CIRAD-BIOS-UMR PVBMT (REU)
• Lett Jean-Michel, CIRAD-BIOS-UMR PVBMT (REU)
• Varsani Arvind, UCT (ZAF)
• Massart Sébastien, GxABT (BEL)
• Ogliastro Mylène, INRAE (FRA)
• Martin Darren Patrick, UCT (ZAF)
• Filloux Denis, CIRAD-BIOS-UMR PHIM (FRA)
• Roumagnac Philippe, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0001-5002-6039

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604140/)

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