Comparison of the signal transduction efficiency in the Vfm quorum sensing system of the genus Dickeya according to the polymorphism of the vfm genes

Yammine Sarah, Boulanger Malo, Hugouvieux-Cotte-Pattat Nicole, Reverchon Sylvie, Cociancich Stéphane, Royer Monique. 2023. Comparison of the signal transduction efficiency in the Vfm quorum sensing system of the genus Dickeya according to the polymorphism of the vfm genes. In : 16th Plant Bacteria Meeting. Abstract Booklet. INRAE. Aussois : INRAE, p. 44. Rencontres plantes-bactéries. 16, Aussois, France, 20 Mars 2023/24 Mars 2023.

Résumé : "The Vfm quorum sensing (QS) system is preponderant for the virulence of different species of the bacterial genus Dickeya. In the model strain D. dadantii 3937, the Vfm QS system was shown to be responsible for the control of the production of plant cell wall degrading enzymes (PCWDE). The transduction of the Vfm QS signal results into the activation of the promoter of the gene vfmE encoding a transcriptional regulator of the AraC family which itself activates the promoter of PCWDE genes [1]. The vfm gene cluster includes 26 genes involved in the biosynthesis, sensing or transduction of the QS signal. It encodes several nonribosomal peptide synthetases (NRPS), indicating that the Vfm QS signal is a complex short peptide. To date, the Vfm QS signal has escaped detection by analytical chemistry methods. Using specific biosensors, a strain-specific polymorphism in the NRPS genes vfmO and vfmP was shown to determine the production of different analogs of the Vfm QS signal [2]. By analogy with the Agr QS system of Staphylococcus aureus, the production of different analogs of the signal is expected to be related to variations in the signal transduction activity of the Vfm QS system, resulting to variations in the level of activation of the promoter of the gene vfmE. To explore this hypothesis, we used a reporter gene fused to the promoter of the gene vfmE to compare the activity of the vfmE promoter among strains of Dickeya producing different analogs of the Vfm QS signal.

Auteurs et affiliations

• Yammine Sarah, CIRAD-BIOS-UMR PHIM (FRA)
• Boulanger Malo, CIRAD-BIOS-UMR PHIM (FRA)
• Hugouvieux-Cotte-Pattat Nicole, INSA de Lyon (FRA)
• Reverchon Sylvie, INSA de Lyon (FRA)
• Cociancich Stéphane, CIRAD-BIOS-UMR PHIM (FRA)
• Royer Monique, CIRAD-BIOS-UMR PHIM (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604263/)

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