Quantification of ASFV DNA and RNA in Ornithodoros soft ticks

Pereira De Oliveira Rémi, Vial Laurence, Le Potier Marie-Frédérique. 2022. Quantification of ASFV DNA and RNA in Ornithodoros soft ticks. In : African swine fever virus: Methods and protocols. Netherton Christopher L. (ed.). New-York : Humana Press, 105-118. (Methods in Molecular Biology, 2503) ISBN 978-1-0716-2332-9

Résumé : Molecular biology methods are highly sensitive to detect the genome of pathogens and to study their biology. Polymerase chain reaction (PCR) and reverse transcription followed by a polymerase chain reaction (RT-PCR) permit the detection of the presence and the replication of African swine fever virus in soft ticks. Here, we described our techniques to detect and quantify DNA and RNA of African swine fever virus in soft ticks including a housekeeping gene of soft ticks as internal control.

Auteurs et affiliations

• Pereira De Oliveira Rémi, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-3005-1277
• Vial Laurence, CIRAD-BIOS-UMR ASTRE (FRA)
• Le Potier Marie-Frédérique, ANSES (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604563/)

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