Identification and validation of QTLs for tuber quality related traits in yam Dioscorea alata

Arnau Gemma, Ehounou Adou Emmanuel, Maledon Erick, Nudol Elie, Vignes Hélène, Gravillon Marie-Claire, N'Guetta Assanvo Simon-Pierre, Mournet Pierre, Chaïr Hâna, Cormier Fabien. 2023. Identification and validation of QTLs for tuber quality related traits in yam Dioscorea alata. In : Proceeding of the International Symposium on Breeding and Effective Use of Biotechnology and Molecular Tools in Horticultural Crops. Bus Vincent (ed.), Causse Mathilde (ed.). ISHS. Louvain : ISHS, 381-388. (Acta Horticulturae, 1362) ISBN 978-94-6261-361-4 International Horticultural Congress (IHC 2022): International Symposium on Breeding and Effective Use of Biotechnology and Molecular Tools in Horticultural Crops. 31, Angers, France, 14 Août 2022/22 Août 2022.

Résumé : Two diploid full-sib segregating populations composed of 193 progenies (population B, 74F x 14M) and 121 progenies (population A, 74F x Kabusa) were used to map several qualitytraits. All progenies and genitors were genotyped-by-sequencing (GBS). A consensus high-density genetic map was constructed containing 15 79 SNP loci, with a total length of 2613.5 cM and an average distance of 1.68 cM between markers. Both populations were assessed over two consecutive years for seven important morpho-agronomie quality-related traits (tuber size, shape regularity, flesh colour, flesh oxidation, oxidation ratio, tubercular roots and skin texture). The heritabilities of most traits were high. Quantitative trait loci (QTLs) were identified for all the traits studied. Thirty-four QTLs were detected on 8 of the 20 yam chromosomes. The fraction of total phenotypic variance attributable to a single QTL ranged from 11.1 to 43.5%. We detected significant correlations between traits and QTL co-localizations that were consistent with these correlations. Nine QTLs were validated on a diversity panel, including three for tuber size, three for shape regularity, two for skin texture and one for tubercular roots. The validated QTLs should be useful for breeding programs using marker-assisted selection to improve yam tuber quality.

Mots-clés libres : Dioscorea alata, Tuber quality, QTLs (quantitative traits loci), Genotyping by sequencing (GBS)

Agences de financement européennes : European Regional Development Fund

Agences de financement hors UE : Région Guadeloupe

Projets sur financement : (FRA) Enhancing Yam Breeding for Increased Productivity and Improved Quality in West Africa, (GLP) Caractérisation et Valorisation de la Biodiversité Végétale Tropicale d’Intérêt Agronomique

Auteurs et affiliations

• Arnau Gemma, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-0714-6830
• Ehounou Adou Emmanuel, CNRA (CIV)
• Maledon Erick, CIRAD-BIOS-UMR AGAP (GLP)
• Nudol Elie, CIRAD-BIOS-UMR AGAP (GLP)
• Vignes Hélène, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-8725-2467
• Gravillon Marie-Claire, CIRAD-BIOS-UMR AGAP (GLP)
• N'Guetta Assanvo Simon-Pierre, UFHB (CIV)
• Mournet Pierre, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8011-8647
• Chaïr Hâna, CIRAD-BIOS-UMR AGAP (FRA)
• Cormier Fabien, CIRAD-BIOS-UMR AGAP (GLP) ORCID: 0000-0002-7283-4616

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604578/)

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