Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species

Chabaud Mireille, Fournier Joëlle, Brichet Lukas, Abdou-Pavy Iltaf, Imanishi Leandro, Brottier Laurent, Pirolles Elodie, Hocher Valérie, Franche Claudine, Bogusz Didier, Wall Luis, Svistoonoff Sergio, Gherbi H., Barker David G.. 2019. Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species. PloS One, 14 (10):e0223149, 13 p.

Quartile : Q2, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intracellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photosynthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria trinervis, actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitotetraose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligosaccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by actinorhizal hosts.

Mots-clés Agrovoc : bactérie fixatrice de l'azote, Frankia, symbiose, spore fongique, plante hôte, histoire naturelle, exsudat de la plante, chitine

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Root hairs, Plants, Symbiosis, Fungi, Legumes, Genetically modified plants, Acetonitrile, Chitin

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Endosymbioses chez les plantes actinorhiziennes : signalisation et mécanismes cellulaires associés à la colonisation racinaire

Auteurs et affiliations

• Chabaud Mireille, INRA (FRA)
• Fournier Joëlle, INRA (FRA)
• Brichet Lukas, INRA (FRA)
• Abdou-Pavy Iltaf, INRA (FRA)
• Imanishi Leandro, IESCT (ARG)
• Brottier Laurent, CIRAD-BIOS-UMR AGAP (FRA)
• Pirolles Elodie, INRA (FRA)
• Hocher Valérie, IRD (FRA)
• Franche Claudine, IRD (FRA)
• Bogusz Didier, IRD (FRA)
• Wall Luis, IESCT (ARG)
• Svistoonoff Sergio, IRD (FRA)
• Gherbi H., IRD (FRA)
• Barker David G., INRA (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/605046/)

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