Minet Cécile, Chantal Isabelle, Thevenon Sophie, Berthier David.
2023. First proof of concept of CRISPR6Cas9 ribonucleoproteins genome editing of Trypanosoma congolense BSF.
. CIRAD
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Version publiée
- Anglais
Accès réservé aux personnels Cirad Utilisation soumise à autorisation de l'auteur ou du Cirad. Symposium nov 2023 Montpellier_ abstract poster_Minet et al.pdf Télécharger (32kB) | Demander une copie |
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Version publiée
- Anglais
Accès réservé aux personnels Cirad Utilisation soumise à autorisation de l'auteur ou du Cirad. Poster_ Minet et al_symposium parasito Mpl 27 28 nov 2023.pdf Télécharger (798kB) | Demander une copie |
Matériel d'accompagnement : 1 poster
Résumé : African Animal Trypanosomosis is a major vector-borne disease affecting livestock breading in Africa. It is caused by trypanosomes, especially Trypanosoma congolense with major negative impacts on animal production and negative repercussions in terms of poverty. To develop new tools to fight against the disease, we need to improve knowledge of the genes involved in the pathogenicity and virulence of the parasites as well as their functions. To date, unlike for Trypanosoma brucei or Trypanosoma cruzi, genome editing tools have been used relatively little to study T. congolense. We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in T. congolense bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins in Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in bloodstream forms of the T. congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens up new perspectives for the control and fight against animal trypanosomosis.
Mots-clés libres : Trypanosoma congolense, African animal trypanosomosis, CRISPR-Cas9, Ribonucleopropteins, Genome editing
Auteurs et affiliations
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Minet Cécile, CIRAD-BIOS-UMR INTERTRYP (FRA)
ORCID: 0000-0002-9287-1481
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Chantal Isabelle, CIRAD-BIOS-UMR INTERTRYP (FRA) ORCID: 0000-0002-3429-484X
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Thevenon Sophie, CIRAD-BIOS-UMR INTERTRYP (FRA) ORCID: 0000-0001-6059-5884
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Berthier David, CIRAD-DG-Direction générale (FRA) ORCID: 0000-0002-3283-6588
Source : Cirad-Agritrop (https://agritrop.cirad.fr/607509/)
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