INDICANTS project : INnovative DIagnostiCs for bANana paThogens Surveillance

Robene Isabelle, Bothma Sheryl, Maillot-Lebon Véronique, Pecrix Yann, Fenelon Babbitha, Matthews Megan, Reynaud Bernard, Coetzee Beatrix, Arrieta Salgado Mikel, Wicker Emmanuel, Chilin-Charles Yolande, Gianinazzi Camilo, Mostert Diane, Viljoen Altus. 2023. INDICANTS project : INnovative DIagnostiCs for bANana paThogens Surveillance. In : 12th International Congress of Plant Pathology (ICPP 2023): One health for all plants, crops and trees. Book of abstracts. ISPP, SFP. Lyon : ISPP, p. 264. International Conference on Plant Pathology (ICPP 2023). 12, Lyon, France, 20 Août 2023/25 Août 2023.

Résumé : The aim of the INDICANTS project is to develop innovative diagnostics for four banana wilt pathogens, including Fusarium oxysporum f. sp. cubense (Foc) TR4 (Fusarium wilt), Ralstonia solanacearum (Moko disease), R. syzygii subsp. celebesensis (Blood disease), and Xanthomonas vasicola pv. musacearum (Xanthomonas wilt). The main objectives are to: (I) develop low-cost LAMP (loop-mediated isothermal amplification) assays (II) compare simplified DNA extraction methods for field application; (III) validate the LAMP protocols via inter-laboratory and field tests. LAMP primer sets were designed for the bacterial pathogens, using in silico comparative genomic analysis of target and non-target genomes, and showed 100% specificity when tested with a wide range of target and non-target strains. A limit of detection of 104 CFU/ml was obtained for the LAMP assays. A simplified DNA extraction method from banana tissue was developed and successfully validated in a banana plantation infested with Foc TR4, using several candidate LAMP primer sets. Ready-to-use diagnostic kits, based on these protocols, are currently being developed by a private company. These point-of-care diagnostic tools will allow rapid identification of the different pathogens in the field for disease management.

Auteurs et affiliations

• Robene Isabelle, CIRAD-BIOS-UMR PVBMT (ZAF)
• Bothma Sheryl, Stellenbosch University (ZAF)
• Maillot-Lebon Véronique, CIRAD-BIOS-UMR PVBMT (REU)
• Pecrix Yann, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-6537-3145
• Fenelon Babbitha, CIRAD-BIOS-UMR PVBMT (REU)
• Matthews Megan, CIRAD-BIOS-UMR PHIM (FRA)
• Reynaud Bernard, Université de la Réunion (REU) ORCID: 0009-0003-6403-3944
• Coetzee Beatrix, Stellenbosch University (ZAF)
• Arrieta Salgado Mikel, CIRAD-BIOS-UMR PHIM (FRA)
• Wicker Emmanuel, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0003-0927-7404
• Chilin-Charles Yolande, CIRAD-BIOS-UMR PHIM (GLP)
• Gianinazzi Camilo, Qualiplante (FRA)
• Mostert Diane, Stellenbosch University (ZAF)
• Viljoen Altus, Stellenbosch University (ZAF)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/607866/)

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