Proteo-Trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae)

Gatti Jean-Luc, Belghazi Maya, Legeai Fabrice, Ravallec Marc, Frayssinet Marie, Robin Stéphanie, Aboubakar Souna Djibril, Srinivasan Ramasamy, Tamo Manuele, Poirié Marylène, Volkoff Anne-Nathalie. 2021. Proteo-Trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae). Toxins, 13 (7), n.spéc. Evolution, Genomics and Proteomics of Venom:502, 36 p.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA733444/ / Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA734452 / Url - autres données associées : https://proteomecentral.proteomexchange.org/

Quartile : Q1, Sujet : TOXICOLOGY / Quartile : Q2, Sujet : FOOD SCIENCE & TECHNOLOGY

Résumé : Meteorus pulchricornis (Ichneumonoidea, Braconidae) is an endoparasitoid wasp of lepidopteran caterpillars. Its parasitic success relies on vesicles (named M. pulchricornis Virus-Like Particles or MpVLPs) that are synthesized in the venom gland and injected into the parasitoid host along with the venom during oviposition. In order to define the content and understand the biogenesis of these atypical vesicles, we performed a transcriptome analysis of the venom gland and a proteomic analysis of the venom and purified MpVLPs. About half of the MpVLPs and soluble venom proteins identified were unknown and no similarity with any known viral sequence was found. However, MpVLPs contained a large number of proteins labelled as metalloproteinases while the most abundant protein family in the soluble venom was that of proteins containing the Domain of Unknown Function DUF-4803. The high number of these proteins identified suggests that a large expansion of these two protein families occurred in M. pulchricornis. Therefore, although the exact mechanism of MpVLPs formation remains to be elucidated, these vesicles appear to be “metalloproteinase bombs” that may have several physiological roles in the host including modifying the functions of its immune cells. The role of DUF4803 proteins, also present in the venom of other braconids, remains to be clarified.

Mots-clés Agrovoc : Meteorus, Braconidae, venin, parasitoïde, Vespidae, Helicoverpa armigera, fonction physiologique

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Meteorus pulchricornis, Parasitoid wasp, Braconidae, Venomics, Virus-like particles (VLPs), Proteomic, Transcriptomic, Metalloproteases, DUF-4803 proteins

Agences de financement hors UE : Agence Nationale de la Recherche, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, French Government

Projets sur financement : (FRA) Adaptation en lutte biologique - Génomique des populations de parasitoïdes, (FRA) Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie, (FRA) Idex UCA JEDI

Auteurs et affiliations

• Gatti Jean-Luc, INRAE (FRA) - auteur correspondant
• Belghazi Maya, CNRS (FRA)
• Legeai Fabrice, INRAE (FRA)
• Ravallec Marc, INRAE (FRA)
• Frayssinet Marie, Université de Montpellier (FRA)
• Robin Stéphanie, INRAE (FRA)
• Aboubakar Souna Djibril, CIRAD-PERSYST-UPR HortSys (BFA)
• Srinivasan Ramasamy, World Vegetable Center (TWN)
• Tamo Manuele, IITA (BEN)
• Poirié Marylène, INRAE (FRA)
• Volkoff Anne-Nathalie, INRAE (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/608248/)

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