NIRS as a high-throughput phenotyping tool for assessing the diversity of leaf functioning under water deficit in a large grapevine panel

Coindre Eva, Ryckewaert Maxime, Chir Laurine, Boulord Romain, Falcon Mélyne, Laisne Thomas, Rolland Gaëlle, Bouckenooghe Virginie, Lis Maëlle, Cabrera-Bosquet Llorenç, Doligez Agnès, Simonneau Thierry, Freitas Virgilio, Thomas Miguel, Pallas Benoît, Coupel-Ledru Aude, Segura Vincent. 2024. NIRS as a high-throughput phenotyping tool for assessing the diversity of leaf functioning under water deficit in a large grapevine panel. In : Résumés des communications présentées aux 25èmes rencontres HélioSPIR, Montpellier (France), 11-12 juin 2024. Bastianelli Denis (ed.), Gilles Chaix (ed.). HélioSPIR. Montpellier : Association HélioSPIR, Résumé, p. 23. Rencontres HélioSPIR. 25, Montpellier, France, 11 Juin 2024/12 Juin 2024.

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Résumé : Water resource is a major limiting factor impacted by climate change, threatening the yield and quality of grapevine production. Understanding the ecophysiological mechanisms involved in response to water deficit is crucial to select new varieties more drought-tolerant. A major bottleneck that hampers such advances is the lack of methods for measuring functioning traits on thousands of leaves as required for genetic analyses. Recent studies have highlighted the interest of near-infrared spectroscopy (NIRS) and chlorophyll fluorescence for high-throughput evaluation of leaf functioning traits. The aim of this study is to develop these methods, and test their robustness to facilitate their deployment for phenotyping the genetic diversity of grapevine. 246 genotypes, representative of the genetic diversity of the species Vitis vinifera, were phenotyped over two consecutive years. In 2021, the genotypes were grown in pots outdoors under non-limiting irrigation conditions, while in 2022, the same potted genotypes were subjected to three different water scenarios (i. Well-watered, ii. Moderate water deficit, iii. Severe water deficit) in a greenhouse (PhenoArch high-throughput phenotyping platform). To evaluate traits related to carbon and water functioning across the entire panel, a subset of genotypes were phenotyped by combining i/ low-throughput devices to precisely measure ecophysiological traits, and ii/ innovative high-throughput portable devices to measure NIRS, porometry and chlorophyll fluorescence. These data enabled the creation of partial least squares regression (PLSR) models using both low- and high-throughput data to predict ecophysiological traits. Leaf mass per area and leaf water content were well predicted by spectrometers (R² > 0.7). Photosynthesis, on the other hand, was well predicted by chlorophyll fluorescence and porometry data. The robustness of the predictive models was tested between experiments by comparing models calibrated with data from one experiment to predict data from the second one. The robustness of the models was dependent on the trait and the high-throughput device used. The prediction of leaf mass per area, using NIRS, appeared to be accurate and stable between experiments. Intra-experiment robustness analysis showed that water deficit can impact the quality of trait predictions, particularly those related to water, such as water content and water use efficiency. The R² and RMSE parameters provided additional information, especially as water deficit affected trait variability. The prediction of these traits was less accurate when applied on a plant that had been grown under severe water deficit. Compelling models will be employed to predict these traits across the entire panel, enabling their use in genetic analysis.

Mots-clés libres : High-throughput phenotyping, Near-infrared spectroscopy, PLS regression, Ecophysiological traits, Water deficit, Model robustness

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