Threshold-dependent improvement in sex separation efficiency by NaCl pretreatment of larval-pupal mixtures in mass-reared Aedes albopictus

Sun Yan, Zhang Junyan, Zheng Keqing, Yamada Hanano, Khan Jehangir, Bouyer Jérémy, Deng Lu, Li Ming, Wu YU, Zheng Xiaoying, Wu Zhongdao, Zhang Dongjing. 2025. Threshold-dependent improvement in sex separation efficiency by NaCl pretreatment of larval-pupal mixtures in mass-reared Aedes albopictus. Pest Management Science, 9 p.

Résumé : BACKGROUND: The removal of female mosquitoes is a critical requirement in sterile insect technique (SIT) for mosquito control. Improving the efficiency and accuracy of sex sorting is therefore vital. Previous studies have demonstrated that treating larval–pupal mixtures with a 15% sodium chloride (NaCl) solution prior to the sex sorting process reduces female contamination in Aedes mosquitoes. However, the effects of NaCl on Aedes albopictus mosquito biology and its practical application in production facilities remain insufficiently explored and warrant further investigation. RESULTS: Exposure of larval–pupal mixtures to 15% NaCl for 60 min led to 100% larval mortality via osmotic imbalance and intestinal tissue damage, while pupae remained unaffected due to their impermeable cuticle. Males emerging from treated pupae exhibited comparable emergence, longevity, and flight ability to controls, indicating no adverse effect on male quality. NaCl pretreatment significantly improved sorting efficiency and reduced female contamination in manual sorting, achieving a 3.9-fold reduction in adult-stage contamination. Although automatic sorters increased throughput, reductions in female contamination were not statistically significant. Importantly, the sorting benefits of NaCl treatment were threshold-dependent and most evident at larger processing scales. CONCLUSION: NaCl pretreatment is a simple, scalable, and biologically safe strategy to enhance sex separation in Ae. albopictus mass production. It is particularly advantageous under high-throughput conditions and preserves male fitness, supporting the stringent quality standards required for successful SIT-based mosquito control. © 2025 Society of Chemical Industry.

Mots-clés Agrovoc : Aedes albopictus, lutte anti-insecte, vecteur de maladie, élevage en masse, Aedes, matériel de triage, pupe, détermination du sexe, lutte biologique, lâcher d'insectes stériles, contrôle de maladies, larve, longévité

Mots-clés libres : Sterile Insect Technique, Sex sorting, Genetic control

Agences de financement hors UE : Key Program of NSFC-Tongyong Union Foundation, Bundesministerium für Gesundheit und Frauen, Basic and Applied Basic Research Foundation of Guangdong Province, Association of International Education Administrators

Auteurs et affiliations

• Sun Yan, Sun Yat-Sen University (CHN)
• Zhang Junyan, Sun Yat-Sen University (CHN)
• Zheng Keqing, Sun Yat-Sen University (CHN)
• Yamada Hanano, FAO (AUT)
• Khan Jehangir, Sun Yat-Sen University (CHN)
• Bouyer Jérémy, CIRAD-BIOS-UMR ASTRE (AUT) ORCID: 0000-0002-1913-416X
• Deng Lu, National Environment Agency (SGP)
• Li Ming, Sun Yat-Sen University (CHN)
• Wu YU, Sun Yat-Sen University (CHN)
• Zheng Xiaoying, Sun Yat-Sen University (CHN)
• Wu Zhongdao, Sun Yat-Sen University (CHN) - auteur correspondant
• Zhang Dongjing, Sun Yat-Sen University (CHN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/614198/)

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