Lanaud Claire, Risterucci Ange-Marie, N'Goran Jeanne A.K., Clément Didier, Flament Marie-Henriette, Laurent V..
1995. A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers.
In : Plant genome III = [Génome des plantes III]. Scherago International
Autre titre : Une carte des liaisons génétiques de Theobroma cacao L. basées sur des isoenzymes et des marqueurs RFLP et RAPD
Résumé : A linkage map of cacao genome have been constructed using isoenzymes, RFLPOs, and RAPDs markers. A genomic library and cDNA library were constructed from germinated seeds of an Amelonado clone. The screening of probes was made on 5 genotypes, using 5 restriction enzymes. Among 587 tested probes, 52% of cDNA and 44% of genomic probes revealed polymorphic patterns. A population of 100 individuals derived from a cross between two heterozygous genotypes has been used. Segregation analyses were performed with JoinMap programm. The map comprised 193 loci: 5 isoenzymes, 101 cDNA/RFLPs, 4 loci from genes of known fonction, 55 genomic DNA/RFLPs and 28 RAPDs. Ten linkage groups, which putatively correspond to the 10 gametic chromosomes of cacao, have been identified. The map covers a total length of 759 cM with a 3,9 cM average distance between two markers. A small fraction (9%) of the markers deviated significantly from the expected mendelian ratios
Mots-clés Agrovoc : Theobroma cacao, adn, carte génétique, RAPD, RFLP, isoenzyme, liaison génétique
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
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Lanaud Claire
ORCID: 0000-0001-6411-7310
- Risterucci Ange-Marie
- N'Goran Jeanne A.K.
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Clément Didier ORCID: 0000-0002-7602-6472
- Flament Marie-Henriette
- Laurent V.
Source : Cirad - Agritrop (https://agritrop.cirad.fr/389712/)
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