RAPD-based screening of genomic libraries for positional cloning

Dioh Waly, Tharreau Didier, Lebrun Marc-Henri. 1997. RAPD-based screening of genomic libraries for positional cloning. Nucleic Acids Research, 25 (24) : 5130-5131.

https://doi.org/10.1093/nar/25.24.5130

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Autre titre : Criblage des banques génomiques par la technique RAPD pour le clonage positionnel

Résumé : RAPD markers are frequently used for positional cloning. However, RAPD markers often contain repeated sequences which prevent genomic library screening by hybridisation. We have developed a simple RAPD analysis of genomic libraries based on the identification of cosmid pools and clones amplifying the RAPD marker of interest. Our method does not require the cloning or characterisation of the RAPD marker as it relies on the analysis of cosmid pools or clones using a simple RAPD protocol. We applied this strategy using four RAPD markers composed of single copy or repeated sequences linked to avirulence genes of the rice blast fungus #Magnaporthe grisea#. Cosmids containing these RAPD markers were easily and rapidly identified allowing the construction of physical contigs at these loci.

Mots-clés Agrovoc : Oryza, Magnaporthe, pouvoir pathogène, génome, carte génétique, RAPD, banque de gènes, maladie de la pyriculariose des plantes

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

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