A major quantitative trait locus for rice yellow mottle virus resistance maps to a cluster of blast resistance genes on chromosome 12

Ghesquière Alain, Albar Laurence, Loriaux M., Ahmadi Nourollah, Fargette Denis, Huang N., McCouch S.R., Nottéghem Jean-Loup. 1997. A major quantitative trait locus for rice yellow mottle virus resistance maps to a cluster of blast resistance genes on chromosome 12. Phytopathology, 87 (12) : 1243-1249.

https://doi.org/10.1094/PHYTO.1997.87.12.1243

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Résumé : Two doubled-haploid rice populations, IR64/Azucena and IRAT177/ Apura, were used to identify marlcers linked to rice yellow mottle virus (RYMV) resistance usina core restriction fragment length polymorphism (RFLP) maps. Resistance was measured by mechanical inoculation of 19-day-old seedlings followed by assessment of virus content by enzymelinked immunosorbent assay tests 15 days after inoculation. IR64/Azucena and IRAT177/Apura populations, 72 and 43 lines, respectively, were evaluated, and resistance was found to be polygenic. Resistance was expressed as a slower virus multiplication, low symptom expression, and limited yield loss when assessed at the field lever. Bulked segregant analysis using the IR64/Azucena population identified a single random amplified polymorphie DNA marker that mapped on chromosome 12 and corresponded to a major quantitative trait locus (QTL) evidenced by interval mapping. When pooling RFLP data, integrated mapping of this chromosome revealed that the QTL was common to the two populations and corresponded to a small chromosomal segment knoun to contain a cluster of major blast resistance genes. This region of the genome also reflected the differentiation observed at the RFLP level between the subspecies #indica# and #japonica# of #Oryza saliva#. This is consistent with the observation that most sources of RYMV resistance used in rice breeding are found in upland rice varieties that typically belong to the #japonica# subspecies.

Mots-clés Agrovoc : sobemovirus marbrure jaune riz, résistance aux maladies, Oryza, gène

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

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