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Application of DNA markers for identification and breeding of citrus rootstocks

Luro François, Ollitrault Patrick. 1999. Application of DNA markers for identification and breeding of citrus rootstocks. In : Proceedings of the 5th World Congress of the International Society of Citrus Nurserymen. Aubert Bernard (ed.). ISCN. Montpellier : CIRAD, pp. 205-212. ISBN 2-87614-337-2 World Congress of the International Society of Citrus Nurserymen. 5, Montpellier, France, 5 March 1997/8 March 1997.

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Autre titre : Application de marqueurs à ADN pour l'identification et l'amélioration des porte greffe d'agrumes

Abstract : The detection and analysis of DNA polymorphisms in crops is an essential component of marker-assisted selection and cultivar identification in plant breeding. We have explored several molecular techniques which allow to detect a DNA polymorphism : isozymes, amplification of microsatellite interspaced DNA sequences (AMIS), random PCR amplified polymorphic DNA (RAPD) and restriction fragment lenght polymorphism (RFLP). Number of problems in citrus breeding and systematics can be approached using these techniques : - molecular markers provide a more direct method to screen a large number of plants, at early development stages; - genetic origin identification (parental origin or nucellar/zygotic origin); - genetic linkage map construction to locate genes involved in the development of agronomically interesting characters; markers associated with these genes could then be used for early selection of progenies and possible progenitors for breeding programs; - in new breeding schemes employing somatic hybridization these molecular markers are essential to control the genetic composition of the vitroplantlets and calli arising from protoplast fusion. Presently, the microsatellite marker seems to be the more valuable tool in genetic studies and appears very promising in all breeding applications. (Résumé d'auteur)

Mots-clés Agrovoc : Citrus, Porte greffe, Amélioration des plantes, ADN, Marqueur génétique, Identification, Polymorphisme génétique, RAPD, RFLP, Isoenzyme, Microsatellite, Carte génétique

Classification Agris : F30 - Plant genetics and breeding
F02 - Plant propagation

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Source : Cirad - Agritrop (https://agritrop.cirad.fr/391223/)

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