Cloning and expression of the binary toxin genes of Bacillus sphaericus C3-41 in a crystal minus B. thuringiensis subsp. Israelensis

Zhiming Y., Neilsen Le Roux C., Pasteur Nicole, Delecluse A., Charles Jean François, Frutos Roger. 1999. Cloning and expression of the binary toxin genes of Bacillus sphaericus C3-41 in a crystal minus B. thuringiensis subsp. Israelensis. Acta Microbiologica Sinica, 39 (1) : 35-39.

Titre chinois : en chinois

Autre titre : Clonage et expression des gènes de la toxine binaire de Bacillus sphaericus C3-41 dans une souche acristallophore de B. thuringiensis subsp. israelensis

Résumé : Baciilus sphearicus C-,-41, belonging o serotype H5a5b, is ahighly toxic strain isolared f,om a mosquito-breeding site in China. It has been shown rhat it had a higher toxiciry against Cu!ex spp. than the reference sirain 2362 at the laboratory and field conditions. Using synthelic ci igonuclect, ides designed on -,he basis of the binary iox;,-, gene sequence of 2362, a 1. lkb DNA fragmeni ,vas produced and the genetic library was prepared from a H;ndIII digest of total DNA from Cy 41 . One colony conzaining the 3 -';kb HincIIII fragment was selected for further studies. Sequence arialysis revealed that ,h;s 3 5kb Hind DNA fragment was composed of 3479 nucleitic adids and the sequence of the binary tox;n gene of C3-41 îs completely identical to that of strain 2362. The toxin geries have been transfer-ed into a nontoxic crystal-minus strain of Bacillus thuringiensis subsp. israeIensis. The recombinant strains could express the binary toxin of B. sphaericus as crystaline structures during their sporulation. The expressilon products of the recombinants have a highly toxiciiv ro susceptible CuZez pipiens subsp. quinquefusciatus and no toxicity to resistant lar-vae( Culex pipie7lS subbp. pipiens) from France.

Mots-clés Agrovoc : Bacillus thuringiensis, gène, Clonage moléculaire, toxine bactérienne, pesticide bactérien, insecticide, expression des gènes

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Zhiming Y.
• Neilsen Le Roux C.
• Pasteur Nicole
• Delecluse A.
• Charles Jean François
• Frutos Roger, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/391978/)

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