Inter-Alu-like species-specific sequences in the Saccharum complex

Alix Karine, Paulet Florence, Glaszmann Jean-Christophe, D'Hont Angélique. 1999. Inter-Alu-like species-specific sequences in the Saccharum complex. Theoretical and Applied Genetics, 99 : 962-968.

https://doi.org/10.1007/s001220051403

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Résumé : #Alu# sequences constitute the most abundant family of short interspersed nuclear elements, SINEs, in the primate genome. The #Alu#-PCR method, which consists of amplification between "Alu# sequences, is usually applied in human genetics to provide polymorphic markers. Here we report the presence of #Alu#-like sequences in sugarcane and related species by applying the #Alu#-PCR-like method. Amplifications using a PCR primer defined in conserved regions of #Alu# human sequences lead to specific complex multiband profiles in all the #Saccharum# and related genera clones surveyed. The isolation and characterisation of the amplified genus-specific inter-#Alu#-like fragments allowed us to isolate repeated sequences that are specific for different genera of the #Saccharum# complex: MsCIR2 from #Miscanthus#, EaCIR6 and EaCIR7 from #Erianthus#, and SrCIR2 from #Saccharum#. Two PCR diagnostic tests were developed from the inter-#Alu#-like sequences MsCIR2 and EaCIR6, and proved efficient in identifying intergeneric hybrids #Saccharum# x #Miscanthus# or #Saccharum# x #Erianthus#, respectively. The present study illustrates how the #Alu#-PCR-like method could help investigating the origin of amphiploid species and monitoring introgression in plants. (Author's abstract)

Mots-clés Agrovoc : Saccharum, séquence nucléotidique, PCR, identification, empreinte ADN

Classification Agris : F30 - Génétique et amélioration des plantes

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