Manohar S.K., Schmitt C., Herzog Etienne, Dollet Michel, Guilley H., Richards K., Jonard G..
1993. Primary structure and genetic organization of Peanut Clump Virus RNA 2.
In : Proceedings of the second symposium of the international working group on plant viruses with fungal vectors. Hiruki C. (ed.)
Autre titre : Structure de base et organisation génétique du virus ARN 2 du clump de l'arachide
Résumé : RNA 2 from an African isolate of the probable furovirus peanut clump virus (PCV) has been cloned and sequenced. The RNA is 4503 nucleotides and contains six long open reading frames (ORFs). The 5'-proximal ORF encodes the coat protein which is followed immediately in another reading frame by an ORF for a 39 kDa protein. Both the coat protein and the 39 kDa protein are expressed from full-length RNA during in vitro translation. Downstream of the 39 kDa ORF is a 'Triple Gene Block' which displays closest homology to the Triple Gene Block of barley stripe mosaic virus. The 3'-proximal ORF encodes a putative 7 kDa protein. Analysis of other PCV isolates has revealed that the 39 kDa ORF can undergo deletion without interfering with infectivity of the virus upon mechanical inoculation to leaves, suggesting that the 39 kDa protein is involved in the natural infection cycle, e.g. fungus transmission. Minor isolate-related sequence changes (nucleotide, substitutions) have also been detected in the coat protein cistron.
Mots-clés Agrovoc : Arachis hypogaea, maladie des plantes, virus des végétaux, identification, arn, Clonage moléculaire, séquence nucléotidique, code génétique
Mots-clés complémentaires : Rabougrissement de l'arachide
Classification Agris : H20 - Maladies des plantes
Auteurs et affiliations
- Manohar S.K.
- Schmitt C.
- Herzog Etienne
- Dollet Michel, CIRAD-CP-DDC (FRA)
- Guilley H.
- Richards K.
- Jonard G.
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/399278/)
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