Identification of pathogenic plant trypanosomes by kinetoplast DNA analysis

Ahomadegbe J.C., Dollet Michel, Gargani Daniel, Riou G.. 1992. Identification of pathogenic plant trypanosomes by kinetoplast DNA analysis. In : Phytomonas workshop = [Second atelier international Phytomonas]. s.l. : s.n., 1 p. International Phytomonas Workshop. 2, Santa Marta, Colombie, 5 Février 1992/8 Février 1992.

Autre titre : Identification des trypanosomes pathogènes des plantes par analyse kinetoplast d'ADN

Résumé : We have extracted and compared the kinetoplast DNA (kDNA) of thirteen #Phytomonas# isolates obtained from different plants originating from various countries and cultured #in vitro#. The kDNA structure from all the isolates appeared as a large network of interlocked minicircles with some maxicircles extruding from the network. The sizes of the minicircles varied from 1.3 to 2.8 kb according to #Phytomonas# isolates. The electrophoretic analysis of the minicircles digested with various restriction endonucleases permified to characterize each #Phytomonas# isolate. Cross hybridization experiments were perfomed by Southern blot techniques using minicircles from different isolates as probes. A high sequence homology was found between minicircles from #Phytomonas# isolated from crude sap of coconut trees affected with Hartrot disease in French Guyana and Brazil. The overall results show that kDNA analysis could be used for distinguishing #Phytomonas# isolates and thus for identifying the infection of plant by pathogenic strain.

Mots-clés Agrovoc : Phytomonas, électrophorèse, analyse biologique, adn

Mots-clés géographiques Agrovoc : France

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Ahomadegbe J.C.
• Dollet Michel
• Gargani Daniel
• Riou G.

Source : Cirad - Agritrop (https://agritrop.cirad.fr/400822/)

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