Histochemical and cytochemical investigations of phenols in roots of banana infected by the burrowing nematode Radopholus similis

Valette Catherine, Andary C., Geiger Jean-Paul, Sarah Jean-Louis, Nicole Michel. 1998. Histochemical and cytochemical investigations of phenols in roots of banana infected by the burrowing nematode Radopholus similis. Phytopathology, 88 (11) : 1141-1148.

Autre titre : Etudes histo- et cytochimiques des phénols dans les racines de bananiers infestés par le nematode Radopholus similis

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : The burrowing nematode #Radopholus similis# is one of the most damaging pathogens on banane plantations. The role of phenolics in plant defense responses to the nematode was histochemically and ultrastructurally investigated in susceptible and partially resistant cultivars. Histochemical observations of healthy roots revealed that high levers of lignin, flavonoids, dopamine, cafeic esters, and ferulic acids were associated with a very low rate of nematode root penetration in the resistant cultivar. The presence of lignified and suberized layers in endodermal cells contributed to limit invasion of the vascular bundle by the pathogen. After infection, flavonoids were seen to accumulate early in walls of cells close to the nematode-migrating channel in both cultivars and in all tissues of the infected resistant roots including the vascular tissues. The labeling pattern obtained with the gold-complexed laccase and with anti-pectin monoclonal antibodies showed that phenolics were distributed in a loosened pectin-rich material surrounding the nematode. This study provides indications that constitutive phenolics in banane roots are associated with the limitation of host penetration and colonization by #R. similis#. Accumulation of flavonoids in response to infection was detected in the vascular tissues of susceptible plants and in all root tissues in the partially resistant plants.

Mots-clés Agrovoc : Radopholus similis, Musa, racine, composé phénolique, analyse histocytologique, ultrastructure, mécanisme de défense, résistance aux organismes nuisibles

Classification Agris : F60 - Physiologie et biochimie végétale
H10 - Ravageurs des plantes

Auteurs et affiliations

• Valette Catherine
• Andary C.
• Geiger Jean-Paul
• Sarah Jean-Louis, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Nicole Michel

Source : Cirad - Agritrop (https://agritrop.cirad.fr/401108/)

Métriques

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